Department of Veterinary Pharmacology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, People's Republic of China.
Int Immunopharmacol. 2011 Dec;11(12):2194-9. doi: 10.1016/j.intimp.2011.09.018. Epub 2011 Oct 20.
Salidroside is a major component isolated from the Rhodiola rosea. In the present study, we investigated the anti-inflammatory effects of salidroside on cytokine production by lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages in vitro, and the results showed that salidroside reduced tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) secretions. This inspired us to further study the effects of salidroside in vivo. Salidroside significantly attenuated TNF-α, IL-1β and IL-6 productions in serum from mice challenged with LPS, and consistent with the results in vitro. In the murine model of endotoxemia, mice were treated with salidroside prior to or after LPS challenge. The results showed that salidroside significantly increased mouse survival. Further studies revealed that salidroside could downregulate LPS-induced nuclear transcription factor-қB (NF-қB) DNA-binding activation and ERK/MAPKs signal transduction pathways production in RAW 264.7 macrophages. These observations indicated that salidroside modulated early cytokine responses by blocking NF-қB and ERK/MAPKs activation, and thus, increased mouse survival. These effects of salidroside may be of potential usefulness in the treatment of inflammation-mediated endotoxemia.
红景天苷是从红景天中分离得到的主要成分。本研究在体外考察了红景天苷对脂多糖（LPS）刺激的 RAW264.7 巨噬细胞细胞因子产生的抗炎作用，结果表明红景天苷能降低肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的分泌。这启发我们进一步研究红景天苷在体内的作用。红景天苷能显著减轻 LPS 刺激的小鼠血清中 TNF-α、IL-1β和 IL-6 的产生，与体外结果一致。在脂多糖内毒素血症的小鼠模型中，用红景天苷对 LPS 攻击前后进行处理。结果表明，红景天苷能显著提高小鼠的存活率。进一步的研究表明，红景天苷能下调 LPS 诱导的核转录因子-κB（NF-κB）DNA 结合活性和 ERK/MAPKs 信号转导途径在 RAW264.7 巨噬细胞中的产生。这些观察结果表明，红景天苷通过阻断 NF-κB 和 ERK/MAPKs 的激活来调节早期细胞因子反应，从而提高了小鼠的存活率。红景天苷的这些作用可能在外源内毒素血症的炎症治疗中有潜在的应用价值。
