Dept. of Biomedical Engineering, College of Engineering, University of Arkansas, Fayetteville, AR, USA.
Biotechnol Prog. 2012 Nov-Dec;28(6):1581-7. doi: 10.1002/btpr.1625. Epub 2012 Sep 27.
We have recently reported on a bench-top approach for isolating extracellular matrix (ECM) from pure populations of cells grown in culture using sacrificial, open-celled foams to concentrate and capture the ECM. To increase both the accumulation and the strength of the ECM harvested, cell-seeded polyurethane (PU) foams were cultured in media supplemented with either transforming growth factor β-1 (TGFβ1) or hepatocyte growth factor (HGF). At the end of a 3-week culture period, ECM yield was significantly increased for samples conditioned in supplemented media. Control foams yielded 48 ± 12 mg of material for every gram of PU foam seeded. Yield values increased to 102 ± 21 and 243 ± 25 mg for HGF and TGFβ1-treated samples, respectively. HGF supplementation increased the modulus by 59%, while TGFβ1 treatment increased the elastic modulus by 204%. TGFβ1-stimulated material was organized into a network that was markedly denser than control material, with HGF-stimulated network density intermediate to TGFβ1 and controls. Our study showed that TGFβ1-treated samples were collagen enriched while HGF samples had an increased gylcosaminoglycan concentration. The results demonstrate that growth factor supplementation, particularly with TGFβ1, can significantly alter the biomechanical properties of cell-derived ECM that may be used for therapeutic applications.
我们最近报道了一种在台面上从纯细胞培养物中分离细胞外基质（ECM）的方法，使用牺牲性、开孔泡沫来浓缩和捕获 ECM。为了增加收获的 ECM 的积累和强度，用转化生长因子β-1（TGFβ1）或肝细胞生长因子（HGF）补充的培养基培养细胞接种的聚氨酯（PU）泡沫。在 3 周的培养期结束时，在补充培养基中培养的样品的 ECM 产量显著增加。对照泡沫每克接种的 PU 泡沫可产生 48 ± 12 mg 的材料。HGF 和 TGFβ1 处理样品的产量值分别增加到 102 ± 21 和 243 ± 25 mg。HGF 补充剂将模量提高了 59%，而 TGFβ1 处理将弹性模量提高了 204%。TGFβ1 刺激的材料组织成一个网络，比对照材料明显更密集，而 HGF 刺激的网络密度介于 TGFβ1 和对照之间。我们的研究表明，TGFβ1 处理的样品富含胶原蛋白，而 HGF 样品的糖胺聚糖浓度增加。结果表明，生长因子补充，特别是 TGFβ1，可以显著改变细胞衍生 ECM 的生物力学特性，可用于治疗应用。
