Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA.
Protein Eng Des Sel. 2012 Dec;25(12):803-11. doi: 10.1093/protein/gzs050. Epub 2012 Aug 22.
Ketoacyl reductases (KRs), hydroxyacyl dehydratases (HDs) and enoyl reductases (ERs) are part of the fatty acid and polyketide synthesis cycles. Their reverse reactions, catalyzed by acyl dehydrogenases (equivalent to ERs), enoyl hydratases (equivalent to HDs) and hydroxyacyl dehydrogenases (equivalent to KRs), are part of fatty acid degradation by β-oxidation. These enzymes have been classified into families based on similarities in their primary and tertiary structures, and these families and their structures are included in the ThYme (Thioester-active enzYmes) database. Members of each family have strong sequence similarity and have essentially the same tertiary structure, mechanism and catalytic residues.
酮酰基还原酶 (KRs)、羟酰基脱水酶 (HDs) 和烯酰基还原酶 (ERs) 是脂肪酸和聚酮合成循环的一部分。它们的逆反应，由酰基脱氢酶（相当于 ERs）、烯酰基水合酶（相当于 HDs）和羟酰基脱氢酶（相当于 KRs）催化，是脂肪酸通过β-氧化降解的一部分。这些酶根据其一级和三级结构的相似性被分类为家族，这些家族及其结构包含在 ThYme（硫酯活性酶）数据库中。每个家族的成员具有很强的序列相似性，并且具有基本相同的三级结构、机制和催化残基。
