长链脂肪酸氧化中烯酰辅酶A水合酶1和烯酰辅酶A水合酶2的比较抑制研究

Comparative inhibition studies of enoyl-CoA hydratase 1 and enoyl-CoA hydratase 2 in long-chain fatty acid oxidation.

作者信息

Wu Long, Lin Shuping, Li Ding

机构信息

Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, P. R. China.

出版信息

Org Lett. 2008 Aug 7;10(15):3355-8. doi: 10.1021/ol801267e. Epub 2008 Jul 9.

DOI:10.1021/ol801267e

PMID:18611036

Abstract

Enoyl-CoA hydratase 1 and enoyl-CoA hydratase 2 in long-chain fatty acid oxidation were comparatively investigated through mechanistic studies for inactivation of the enzymes with methylenecyclopropylformyl-CoA and 3-octynoyl-CoA. Methylenecyclopropylformyl-CoA can inactivate both enzymes, while 3-octynoyl-CoA inactivates enoyl-CoA hydratase 2 only. The study increased our understanding of these two enzymes in fatty acid oxidation.

摘要

通过用亚甲基环丙基甲酰辅酶A和3-辛炔酰辅酶A使酶失活的机理研究，对长链脂肪酸氧化中的烯酰辅酶A水合酶1和烯酰辅酶A水合酶2进行了比较研究。亚甲基环丙基甲酰辅酶A可使这两种酶失活，而3-辛炔酰辅酶A仅使烯酰辅酶A水合酶2失活。该研究增进了我们对脂肪酸氧化中这两种酶的理解。