Wardley R C, Chapman W G, Garland A J
J Hyg (Lond). 1979 Dec;83(3):507-12. doi: 10.1017/s0022172400026358.
A blastogenic test to detect peripheral blood leukocytes specifically sensitized to foot-and-mouth disease virus antigen is described. The test is carried out in microtitre plates and optimum conditions were found by titration. These employed 7.5 x 10(5) cells/well and 20 complement fixing units of antigen. Peak [3H]thymidine incorporation was found to take place at 2-3 days.
本文描述了一种用于检测对口蹄疫病毒抗原产生特异性致敏的外周血白细胞的增殖试验。该试验在微量滴定板中进行,并通过滴定确定了最佳条件。这些条件包括每孔7.5×10⁵个细胞和20个补体结合单位的抗原。发现[³H]胸腺嘧啶核苷掺入峰值出现在2 - 3天。