Department of Oral Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 Project, Chonbuk National University, Jeonju, Republic of Korea.
Anim Reprod Sci. 2012 Oct;134(3-4):164-76. doi: 10.1016/j.anireprosci.2012.07.008. Epub 2012 Jul 31.
Information obtained from peripheral blood could help us understand the underlying mechanisms in autoimmune diseases, cancer, pregnancy, and other conditions. In this paper, we present the protein map of porcine peripheral blood mononuclear cells (PBMC) to better understand the molecular expression changes that occur during pregnancy using proteomic analysis. We detected 94 differentially expressed proteins in pregnant vs. non-pregnant (NP) pigs, and a representative set of the proteins was subjected to LC-MS/MS analysis. Furthermore, the identified proteins were categorized according to their biological process and molecular function. By classifying the proteins according to their functions, a large number of differentially regulated proteins involved in anti-oxidant, detoxification and stress response pathways were found, including peroxiredoxin (PRX) 1, 2, and 6, glutathione-S-transferase (GST), annexin A2, and A6, and heat shock protein 27 (HSP 27) during pregnancy (pregnancy d of E40, embryonic day 40; E70, embryonic day 70; and E93, embryonic day 93) compared with non-pregnancy. In this study, a proteomic approach utilizing 2-DE and LC-MS/MS was applied to evaluate specific molecular expression changes during pregnancy compared with non-pregnancy. Together, these data offer new information about the proteome map and factors that are differentially regulated during maintenance of normal pregnancy.
外周血中获得的信息可以帮助我们了解自身免疫性疾病、癌症、妊娠和其他疾病的潜在机制。在本文中，我们展示了猪外周血单个核细胞（PBMC）的蛋白质图谱，以便通过蛋白质组学分析更好地了解妊娠期间发生的分子表达变化。我们检测到妊娠与非妊娠（NP）猪之间有 94 种差异表达的蛋白质，对其中一组有代表性的蛋白质进行了 LC-MS/MS 分析。此外，根据其生物学过程和分子功能对鉴定出的蛋白质进行了分类。通过根据功能对蛋白质进行分类，发现了大量参与抗氧化、解毒和应激反应途径的差异调节蛋白，包括过氧化物酶（PRX）1、2 和 6、谷胱甘肽-S-转移酶（GST）、膜联蛋白 A2 和 A6，以及热休克蛋白 27（HSP 27），这些蛋白在妊娠（E40、E70 和 E93）与非妊娠时相比有差异（E40、E70 和 E93 分别为胚胎第 40 天、第 70 天和第 93 天）。在这项研究中，利用 2-DE 和 LC-MS/MS 的蛋白质组学方法用于评估与非妊娠相比妊娠期间的特定分子表达变化。这些数据共同提供了有关正常妊娠维持期间差异调节的蛋白质组图谱和因素的新信息。
