Degroote Roxane L, Hauck Stefanie M, Amann Barbara, Hirmer Sieglinde, Ueffing Marius, Deeg Cornelia A
Institute of Animal Physiology, Department of Veterinary Sciences, Ludwig Maximilians University Munich, Munich, Germany.
Research Unit Protein Sciences, Helmholtz Center Munich, German Research Center for Environmental Health, Neuherberg, Germany.
PLoS One. 2014 Mar 10;9(3):e91684. doi: 10.1371/journal.pone.0091684. eCollection 2014.
Equine recurrent uveitis is a spontaneous, lymphocyte-driven autoimmune disease. It affects horses worldwide and presents with painful remitting-relapsing inflammatory attacks of inner eye structures eventually leading to blindness. Since lymphocytes are the key players in equine recurrent uveitis, we were interested in potential changes of their protein repertoire which may be involved in disease pathogenesis. To create a reference for differential proteome analysis, we first unraveled the equine lymphocyte proteome by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequently identified 352 protein spots. Next, we compared lymphocytes from ERU cases and healthy horses with a two-dimensional fluorescence difference in gel electrophoresis approach. With this technique, we identified seven differentially expressed proteins between conditions. One of the significantly lower expressed candidates, septin 7, plays a role in regulation of cell shape, motility and migration. Further analyses revealed T cells as the main cell type with decreased septin 7 abundance in equine recurrent uveitis. These findings point to a possible pathogenetic role of septin 7 in this sight-threatening disease.
马复发性葡萄膜炎是一种自发性的、由淋巴细胞驱动的自身免疫性疾病。它影响着全球的马匹，表现为眼内结构反复出现疼痛性的炎症发作，最终导致失明。由于淋巴细胞是马复发性葡萄膜炎的关键因素，我们对其蛋白质组库中可能参与疾病发病机制的潜在变化感兴趣。为了为差异蛋白质组分析创建一个参考，我们首先通过二维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳解析马淋巴细胞蛋白质组，随后鉴定出352个蛋白质斑点。接下来，我们采用二维荧光差异凝胶电泳方法比较了马复发性葡萄膜炎病例和健康马匹的淋巴细胞。通过这项技术，我们在不同条件下鉴定出了7种差异表达的蛋白质。其中一个表达显著降低的候选蛋白，即septin 7，在细胞形状、运动性和迁移的调节中发挥作用。进一步分析表明，T细胞是马复发性葡萄膜炎中septin 7丰度降低的主要细胞类型。这些发现表明septin 7在这种威胁视力的疾病中可能具有致病作用。
