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胎儿宫内生长受限的羊水和脐血血清蛋白质组学特征的变化。

Changes in amniotic fluid and umbilical cord serum proteomic profiles of foetuses with intrauterine growth retardation.

机构信息

Dipartimento di Biotecnologie, Laboratorio di Proteomica e Spettrometria di massa, Università degli Studi di Verona, Verona, Italy.

出版信息

Electrophoresis. 2011 Dec;32(24):3630-7. doi: 10.1002/elps.201100256.

Abstract

Foetal growth is a result of a complex net of processes, requiring coordination within the maternal, placental, and foetal compartments, the imbalance or lack of which may lead to intrauterine growth restriction (IUGR). IUGR is the major cause of perinatal morbidity and mortality, and is also related to enhanced morbidity and metabolic abnormalities later in life. In the present study, the protein profiles of umbilical cord serum (UCS) and amniotic fluid (AF) of ten IUGR and ten appropriate for gestational age newborns have been analysed by 2-DE, and nanoHPLC-Chip/MS technology. A total of 18 and 13 spots were found to be differentially expressed (p<0.01) in UCS and AF respectively. The unique differentially expressed proteins identified by MS/MS analysis were 14 in UCS, and 11 in AF samples. Protein gene ontology classification indicate that 21% of proteins are involved in inflammatory response, 20% in immune response, while a smaller proportion are related to transport, blood pressure, and coagulation. These results support the conclusion that the IUGR condition alters the expression of proteins involved in the coagulation process, immune mechanisms, blood pressure and iron and copper homeostasis control, offering a new insight into IUGR pathogenesis.

摘要

胎儿生长是一个复杂的过程网络的结果,需要在母体、胎盘和胎儿隔室之间进行协调,这种平衡的失调或缺乏可能导致宫内生长受限(IUGR)。IUGR 是围产期发病率和死亡率的主要原因,也与生命后期发病率和代谢异常的增加有关。在本研究中,通过 2-DE 和纳升 HPLC-Chip/MS 技术分析了 10 例 IUGR 和 10 例适合胎龄新生儿的脐带血清(UCS)和羊水(AF)的蛋白质图谱。在 UCS 和 AF 中分别发现了 18 个和 13 个差异表达的斑点(p<0.01)。通过 MS/MS 分析鉴定的独特差异表达蛋白在 UCS 中有 14 个,在 AF 样本中有 11 个。蛋白质基因本体分类表明,21%的蛋白质参与炎症反应,20%参与免疫反应,而较小比例的蛋白质与运输、血压和凝血以及铁铜稳态控制有关。这些结果支持 IUGR 状况改变了参与凝血过程、免疫机制、血压和铁铜稳态控制的蛋白质表达的结论,为 IUGR 的发病机制提供了新的见解。

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