Taniyama T, Holden H T
J Exp Med. 1979 Dec 1;150(6):1367-82. doi: 10.1084/jem.150.6.1367.
We have developed a system to induce oncornavirus-specific secondary cytotoxic response in vitro. When Moloney strain of murine sarcoma virus-immune spleen cells were cultivated with purified infectious Moloney murine leukemia virus (M-MuLV) or with supernates of tissue culture cells containing infectious virus, a virus-specific secondary cytotoxic response directed against type-specific determinant(s) of M-MuLV was generated in vitro, as determined by a 4-h 51Cr-release assay. The effector cells were susceptible to the treatment with anti-Thyl.2 plus complement, but were unrelated to natural killer cells (NK), because they could not lyse some target cells specific for M-MuLV in both the induction phase and the interaction between effector cells and target cells. Furthermore, a product of the env gene of M-MuLV, perhaps gp70, appeared to be responsible for this response, because viruses with recombinations in the env gene between ecotropic M-MuLV and a xenotropic virus failed to induce a response. When infectious M-MuLV was exposed to UV-light at different doses, the ability of UV-treated M-MuLV to induce a secondary cytotoxic response decreased in parallel with infectivity, indicating that infectivity was necessary for the induction of this response.
我们已经开发出一种在体外诱导肿瘤病毒特异性二次细胞毒性反应的系统。当用纯化的感染性莫洛尼鼠白血病病毒（M-MuLV）或含有感染性病毒的组织培养细胞上清液培养莫洛尼株鼠肉瘤病毒免疫的脾细胞时，通过4小时51Cr释放试验测定，在体外产生了针对M-MuLV型特异性决定簇的病毒特异性二次细胞毒性反应。效应细胞易受抗Thyl.2加补体的处理，但与自然杀伤细胞（NK）无关，因为它们在诱导阶段以及效应细胞与靶细胞之间的相互作用中均不能裂解某些对M-MuLV特异的靶细胞。此外，M-MuLV的env基因产物，可能是gp70，似乎对此反应负责，因为嗜亲性M-MuLV与异嗜性病毒之间在env基因中发生重组的病毒未能诱导出反应。当将感染性M-MuLV暴露于不同剂量的紫外线下时，经紫外线处理的M-MuLV诱导二次细胞毒性反应的能力与感染性平行下降，表明感染性是诱导此反应所必需的。
