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通过聚合酶链反应快速检测急性淋巴细胞白血病和慢性粒细胞白血病中的嵌合bcr/abl mRNA

Rapid detection of chimeric bcr/abl mRNAs in acute lymphoblastic and chronic myeloid leukemia by the polymerase chain reaction.

作者信息

Maurer J, Thiel E

机构信息

Department of Hematology and Oncology, Universitätsklinikum Steglitz, Free University of Berlin, Federal Republic of Germany.

出版信息

Blut. 1990 Dec;61(6):350-3. doi: 10.1007/BF01738548.

DOI:10.1007/BF01738548
PMID:2291982
Abstract

We have developed a rapid method for the detection of bcr/abl mRNAs, the products of the BCR/ABL fusion genes. The method is based on the polymerase-chain-reaction (PCR). Through the use of additional internal primers it is possible to detect directly a single Ph1-positive cell among 10(5) unaffected cells thus omitting time-consuming blotting procedures. The whole analytical procedure starting from RNA isolation to agarose gel electrophoresis including two rounds of PCR can be performed in less than six hours.

摘要

我们已经开发出一种快速检测bcr/abl信使核糖核酸（mRNA）的方法，该mRNA是BCR/ABL融合基因的产物。此方法基于聚合酶链反应（PCR）。通过使用额外的内部引物，能够直接在10⁵个未受影响的细胞中检测出单个Ph1阳性细胞，从而省去了耗时的印迹程序。从RNA分离到琼脂糖凝胶电泳，包括两轮PCR的整个分析过程可在不到6小时内完成。

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Rapid detection of chimeric bcr/abl mRNAs in acute lymphoblastic and chronic myeloid leukemia by the polymerase chain reaction.通过聚合酶链反应快速检测急性淋巴细胞白血病和慢性粒细胞白血病中的嵌合bcr/abl mRNA
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本文引用的文献

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Philadelphia chromosomal breakpoints are clustered within a limited region, bcr, on chromosome 22.费城染色体断点聚集在22号染色体上一个有限的区域——bcr内。
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Ph1-positive leukaemia, including chronic myelogenous leukaemia.Ph1 阳性白血病，包括慢性粒细胞白血病。
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Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.DNA的体外特异性酶促扩增：聚合酶链反应
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Alternative splicing of RNAs transcribed from the human abl gene and from the bcr-abl fused gene.从人类abl基因和bcr-abl融合基因转录的RNA的可变剪接。
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