Peter Grünberg Institute/Institute of Complex Systems Bioelectronics (PGI-8/ICS-8), Research Center Juelich, D-52425 Juelich, Germany.
Langmuir. 2012 Sep 18;28(37):13387-94. doi: 10.1021/la302500r. Epub 2012 Sep 6.
The supported lipid bilayer (SLB) is a well-known system for studying the cell membrane and membrane proteins. It is also promising as a platform for studying cell processes: the cell adhesion, the cell membrane receptors, and the intercellular signaling processes. SLBs made of natural lipids appeared to be protein and cell repellent. Thus, to use the SLB as a substrate for cells, one should functionalize them to provide adhesion. In the present paper, we describe a simple approach to promote adhesion of neuronal cells to the SLB without using proteins or peptides, by introducing positively charged lipids 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) into the SLB made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). We show that neurons adhere to these bilayers and grow for at least 10 days. The SLBs themselves were found to degrade with time in cell culture conditions, but maintained fluidity (as revealed by fluorescence recovery after photobleaching), demonstrating the possibility of using SLBs for studying neuronal cells in culture.
支持的脂质双层（SLB）是研究细胞膜和膜蛋白的知名系统。它也有望成为研究细胞过程的平台：细胞黏附、细胞膜受体和细胞间信号转导过程。由天然脂质组成的 SLB 似乎对蛋白质和细胞具有排斥性。因此，要将 SLB 用作细胞的基质，就应该对其进行功能化以提供黏附性。在本文中，我们描述了一种简单的方法，通过在由 1-棕榈酰基-2-油酰基-sn-甘油-3-磷酸胆碱（POPC）制成的 SLB 中引入带正电荷的脂质 1,2-二油酰基-3-三甲铵丙烷（DOTAP），来促进神经元细胞黏附到 SLB 上，而无需使用蛋白质或肽。我们表明神经元可以黏附在这些双层膜上并至少生长 10 天。在细胞培养条件下，随着时间的推移发现 SLB 本身会降解，但仍保持流动性（如荧光恢复后光漂白所揭示的），证明了在培养中使用 SLB 研究神经元细胞的可能性。
