利用高通量测序和降解组分析鉴定油菜中的 microRNAs 及其靶标。
Identification of miRNAs and their targets from Brassica napus by high-throughput sequencing and degradome analysis.
机构信息
Biotechnology Research Institute, National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
出版信息
BMC Genomics. 2012 Aug 24;13:421. doi: 10.1186/1471-2164-13-421.
BACKGROUND
MicroRNAs (miRNAs) are endogenous regulators of a broad range of physiological processes and act by either degrading mRNA or blocking its translation. Oilseed rape (Brassica napus) is one of the most important crops in China, Europe and other Asian countries with publicly available expressed sequence tags (ESTs) and genomic survey sequence (GSS) databases, but little is known about its miRNAs and their targets. To date, only 46 miRNAs have been identified in B. napus.
RESULTS
Forty-one conserved and 62 brassica-specific candidate B. napus miRNAs, including 20 miRNA* sequences, were identified using Solexa sequencing technology. Furthermore, 33 non-redundant mRNA targets of conserved brassica miRNAs and 19 new non-redundant mRNA targets of novel brassica-specific miRNAs were identified by genome-scale sequencing of mRNA degradome.
CONCLUSIONS
This study describes large scale cloning and characterization of B. napus miRNAs and their potential targets, providing the foundation for further characterization of miRNA function in the regulation of diverse physiological processes in B. napus.
背景
MicroRNAs（miRNAs）是广泛生理过程的内源性调节剂，通过降解 mRNA 或阻止其翻译来发挥作用。油菜（Brassica napus）是中国、欧洲和其他亚洲国家最重要的作物之一，具有公开可用的表达序列标签（EST）和基因组普查序列（GSS）数据库，但对其 miRNA 及其靶标知之甚少。迄今为止，仅在 B. napus 中鉴定出 46 个 miRNAs。
结果
使用 Solexa 测序技术鉴定了 41 个保守和 62 个 Brassica 特异性候选 B. napus miRNAs，包括 20 个 miRNA*序列。此外，通过 mRNA 降解组的基因组规模测序鉴定了 33 个保守 Brassica miRNAs 的非冗余 mRNA 靶标和 19 个新的 Brassica 特异性 miRNA 的非冗余 mRNA 靶标。
结论
本研究描述了 B. napus miRNAs 及其潜在靶标的大规模克隆和表征，为进一步研究 miRNA 在 B. napus 中调节各种生理过程中的功能提供了基础。
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