Department of Biotechnology, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
Food Chem Toxicol. 2012 Nov;50(11):4191-8. doi: 10.1016/j.fct.2012.08.015. Epub 2012 Aug 17.
The purpose of this study was to evaluate the antioxidative activities of water and 70% ethanolic extracts from the Thymus quinquecostatus Celak (TQC) for natural antioxidant source. The antioxidant activities were compared with other natural and synthetic antioxidants. The levels of total polyphenols and flavonoids were also determined. The extracts were found to have different levels of antioxidant properties in a few kind of assay. The results showed that higher radical scavenging activity, reducing power and antioxidant capacity in FRAP than those of BHT as a positive control. In addition, the extracts from the TQC leaf and stem showed stronger antioxidant activity than that of vitamin C, α-tocopherol in ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods. Cytoprotective and anti-apoptotic effect of water extracts from TQC was also prevented t-BHP-induced toxicity in Chang liver cells. Therefore, these results indicate that TQC extracts have antioxidant properties through its ability to enhance the cell viability, reduction of production of ROS, inhibition of oxidative damage, mitochondria dysfunction and ultimately inhibition of cell apoptosis. Based on the results described above, it is suggested that TQC has the potential to protect liver on t-BHP-induced cell damage and should be considered as a prospective functional food.
本研究旨在评估百里香（TQC）的水提物和 70%乙醇提取物的抗氧化活性，以寻找天然抗氧化剂来源。将其抗氧化活性与其他天然和合成抗氧化剂进行了比较。同时还测定了总多酚和类黄酮的含量。在几种测定方法中，提取物表现出不同程度的抗氧化特性。结果表明，提取物的自由基清除活性、还原能力和 FRAP 抗氧化能力均高于阳性对照 BHT。此外，与 FTC 和 TBA 法中的维生素 C、α-生育酚相比，TQC 叶和茎的提取物表现出更强的抗氧化活性。TQC 水提取物还具有细胞保护和抗细胞凋亡作用，可预防 t-BHP 诱导的 Chang 肝细胞毒性。因此，这些结果表明 TQC 提取物具有抗氧化特性，可通过增强细胞活力、减少 ROS 生成、抑制氧化损伤、线粒体功能障碍和最终抑制细胞凋亡来实现。基于上述结果，建议 TQC 具有保护肝脏免受 t-BHP 诱导的细胞损伤的潜力，可将其视为有前景的功能性食品。
