Division of Virology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme-XM, Beliaghata, Kolkata 700 010, West Bengal, India.
Infect Genet Evol. 2012 Dec;12(8):1685-93. doi: 10.1016/j.meegid.2012.07.018. Epub 2012 Aug 11.
Human astroviruses (HAstVs) have now emerged as another common cause of non-bacterial acute gastroenteritis (AGE) in humans worldwide. This study investigated the epidemiology and genetic diversity of human astrovirus strains circulating among infants, younger children (up to 6 years), older children and adolescents (>6-17 years) and adults (18 years and above) hospitalized for diarrhea and their role in AGE in Kolkata, India. A total of 2535 fecal samples were screened for the presence of known enteric viral, bacterial and parasitic etiologies by conventional microbiological assays and molecular methods. The overall incidences of sole or mixed infection of HAstV with known enteric viral, bacterial and parasitic pathogens were detected in 60 cases (2.4%) among all age groups. The clinical symptoms of astrovirus-associated acute watery diarrhea cases were recorded for all sole and mixed infection cases. A high number of sole (n = 13/60 [21.7%]) and mixed infection cases (n = 22/60 [36.7%]) were observed in adults (18 years old or more). Considering all age groups, 18 sole infection cases (n = 18/60 [30%]) and 42 mixed infection cases (n = 42/60 [70%]) with Rotavirus (n = 11/25 [44%]), Vibrio cholerae O1 (n = 6/24 [25%]) Cryptosporidium spp and Giardia lamblia (n = 5/13 [38.4%]) were observed. Further, eleven HAstV samples from infants and children (up to 6 years), children and adolescents (>6-17 years) and adults (18 years and above) were analyzed for their sequences of overlap region between ORF1b (RdRp) and ORF2 (capsid). Among these, ten strains were found to have close genetic relatedness to the Japanese strain HAstV_G1 [AB009985]. Additionally, the IDH2211 Kolkata strain showed a close genetic match with the Thai HAstV_G3 strain [EU363889]. Our study reports show that HAstVs as the sole agent and as mixed infection with other known enteric viral, bacterial, parasitic pathogens are also responsible for AGE among infants, children, adolescents and adults in Kolkata, India.
人类星状病毒（HAstV）现已成为全球范围内导致非细菌性急性胃肠炎（AGE）的另一个常见病因。本研究调查了在印度加尔各答因腹泻住院的婴儿、幼儿（6 岁以下）、儿童和青少年（6-17 岁）以及成年人（18 岁及以上）中循环的人类星状病毒株的流行病学和遗传多样性及其在 AGE 中的作用。通过常规微生物学检测和分子方法，共筛选了 2535 份粪便样本，以检测已知的肠病毒、细菌和寄生虫病因。在所有年龄组中，检测到 60 例（2.4%）仅或混合感染 HAstV 与已知肠病毒、细菌和寄生虫病原体。记录了所有单独或混合感染星状病毒引起的急性水样腹泻病例的临床症状。在成年人（18 岁或以上）中，单独感染（n=13/60[21.7%]）和混合感染（n=22/60[36.7%]）的病例数量较多。考虑到所有年龄组，18 例单独感染病例（n=18/60[30%]）和 42 例混合感染病例（n=42/60[70%]）与轮状病毒（n=11/25[44%]）、霍乱弧菌 O1（n=6/24[25%]）、隐孢子虫和蓝氏贾第鞭毛虫（n=5/13[38.4%]）同时感染。此外，还对来自婴儿和儿童（6 岁以下）、儿童和青少年（6-17 岁）以及成年人（18 岁及以上）的 11 株 HAstV 样本进行了其 ORF1b（RdRp）和 ORF2（衣壳）之间重叠区域序列分析。其中，10 株与日本株 HAstV_G1[AB009985]密切相关。此外，IDH2211 加尔各答株与泰国 HAstV_G3 株密切匹配[EU363889]。我们的研究报告显示，HAstV 作为单一病原体以及与其他已知肠病毒、细菌、寄生虫病原体的混合感染也是印度加尔各答婴儿、儿童、青少年和成年人 AGE 的病因。
