Department of Pediatrics, Dokuz Eylul University Medical Faculty, İzmir, Turkey.
Urology. 2012 Nov;80(5):1161.e7-12. doi: 10.1016/j.urology.2012.06.046. Epub 2012 Aug 24.
To evaluate the efficiency of mesenchymal stem cells in ameliorating renal scarring in a rat pyelonephritis model.
Three groups each, including 8 Sprague-Dawley rats were formed: Group 1 = sham operated (4 were given mesenchymal stem cells); group 2 = pyelonephritis induced by Escherichia coli; and group 3 = pyelonephritis and mesenchymal stem cells. Rats not given mesenchymal stem cells in group 1 and 4 rats in groups 2 and 3 were sacrificed on the eighth day for evaluation of inflammation, and the remaining rats were sacrificed at the sixth week to determine renal scarring along with migration of mesenchymal stem cells to renal tubules and differentiation to tubular cells expressing aquaporin-1.
Rats in group 3 had lower scores of both acute (8th day) and chronic (6th week) histopathological alterations compared with rats in group 2. By contrast, although rats in group 3 were shown to have mesenchymal stem cells expressing aquaporin-1 in their renal tubules, these cells were not detected in kidney tissue of mesenchymal stem cells-treated sham rats.
These results indicate that mesenchymal stem cells migrated to renal tissues and ameliorated renal scarring in this rat model of pyelonephritis.
评估间充质干细胞改善肾盂肾炎大鼠肾瘢痕形成的效果。
每组 8 只 Sprague-Dawley 大鼠分为 3 组：1 组 = 假手术（4 只给予间充质干细胞）；2 组 = 大肠杆菌诱导的肾盂肾炎；3 组 = 肾盂肾炎和间充质干细胞。第 1 组和第 4 组未给予间充质干细胞的大鼠和第 2 组和第 3 组的 4 只大鼠在第 8 天处死以评估炎症，其余大鼠在第 6 周处死以确定肾瘢痕形成以及间充质干细胞向肾小管迁移和分化为表达水通道蛋白-1 的肾小管细胞。
与第 2 组相比，第 3 组大鼠的急性（第 8 天）和慢性（第 6 周）组织病理学改变评分均较低。相比之下，虽然第 3 组大鼠的肾组织中可见表达水通道蛋白-1 的间充质干细胞，但在给予间充质干细胞的假手术大鼠的肾组织中未检测到这些细胞。
这些结果表明，间充质干细胞迁移到肾组织中，并改善了肾盂肾炎大鼠的肾瘢痕形成。
