Chimenti Isotta, Forte Elvira, Angelini Francesco, Messina Elisa, Giacomello Alessandro
Department of Medical Surgical Sciences and Biotechnology, Sapienza University, Italy.
Biochim Biophys Acta. 2013 Feb;1830(2):2459-69. doi: 10.1016/j.bbagen.2012.08.004. Epub 2012 Aug 21.
Cardiac regenerative medicine is a rapidly evolving field, with promising future developments for effective personalized treatments. Several stem/progenitor cells are candidates for cardiac cell therapy, and emerging evidence suggests how multiple metabolic and biochemical pathways strictly regulate their fate and renewal.
In this review, we will explore a selection of areas of common interest for biology and biochemistry concerning stem/progenitor cells, and in particular cardiac progenitor cells. Numerous regulatory mechanisms have been identified that link stem cell signaling and functions to the modulation of metabolic pathways, and vice versa. Pharmacological treatments and culture requirements may be exploited to modulate stem cell pluripotency and self-renewal, possibly boosting their regenerative potential for cell therapy.
Mitochondria and their many related metabolites and messengers, such as oxygen, ROS, calcium and glucose, have a crucial role in regulating stem cell fate and the balance of their functions, together with many metabolic enzymes. Furthermore, protein biochemistry and proteomics can provide precious clues on the definition of different progenitor cell populations, their physiology and their autocrine/paracrine regulatory/signaling networks.
Interdisciplinary approaches between biology and biochemistry can provide productive insights on stem/progenitor cells, allowing the development of novel strategies and protocols for effective cardiac cell therapy clinical translation. This article is part of a Special Issue entitled Biochemistry of Stem Cells.
心脏再生医学是一个快速发展的领域，在有效的个性化治疗方面有着广阔的未来发展前景。几种干细胞/祖细胞是心脏细胞治疗的候选者，新出现的证据表明多种代谢和生化途径如何严格调控它们的命运和更新。
在本综述中，我们将探讨生物学和生物化学在干细胞/祖细胞，特别是心脏祖细胞方面共同感兴趣的一些领域。已经确定了许多调控机制，这些机制将干细胞信号传导和功能与代谢途径的调节联系起来，反之亦然。可以利用药物治疗和培养条件来调节干细胞的多能性和自我更新能力，可能会增强它们在细胞治疗中的再生潜力。
线粒体及其众多相关代谢物和信使，如氧气、活性氧、钙和葡萄糖，与许多代谢酶一起，在调节干细胞命运及其功能平衡方面起着关键作用。此外，蛋白质生物化学和蛋白质组学可以为不同祖细胞群体的定义、它们的生理学以及它们的自分泌/旁分泌调节/信号网络提供宝贵线索。
生物学和生物化学之间的跨学科方法可以为干细胞/祖细胞提供富有成效的见解，从而开发出用于有效的心脏细胞治疗临床转化的新策略和方案。本文是名为“干细胞生物化学”的特刊的一部分。
