酵母中外源乙醇的代谢反应：一种体内统计全相关 NMR 光谱学方法。

Metabolic response to exogenous ethanol in yeast: an in vivo statistical total correlation NMR spectroscopy approach.

机构信息

Department of Pharmaceutical and Applied Chemistry, University of Siena, Siena, Italy.

出版信息

J Biosci. 2012 Sep;37(4):749-55. doi: 10.1007/s12038-012-9237-z.

DOI:10.1007/s12038-012-9237-z

Abstract

In vivo NMR spectroscopy, together with selectively 13C-labelled substrates and 'statistical total correlation spectroscopy' analysis (STOCSY), are valuable tools to collect and interpret the metabolic responses of a living organism to external stimuli. In this study, we applied this approach to evaluate the effects of increasing concentration of exogenous ethanol on the Saccharomyces cerevisiae fermentative metabolism. We show that the STOCSY analysis correctly identifies the different types of correlations among the enriched metabolites involved in the fermentation, and that these correlations are quite stable even in presence of a stressing factor such as the exogenous ethanol.

摘要

体内 NMR 光谱学，与选择性 13C 标记的底物和“统计全相关光谱学”分析（STOCSY）一起，是收集和解释生物体对外界刺激的代谢反应的有价值的工具。在这项研究中，我们应用这种方法来评估外源性乙醇浓度增加对酿酒酵母发酵代谢的影响。我们表明，STOCSY 分析正确地识别了发酵过程中富集代谢物之间的不同类型的相关性，并且即使存在应激因素（如外源性乙醇），这些相关性也相当稳定。