Department of Pharmaceutical and Applied Chemistry, University of Siena, Via Aldo Moro, 2-53100 Siena, Italy.
J Biosci. 2011 Mar;36(1):97-103. doi: 10.1007/s12038-011-9003-7.
Mathematical modelling analysis of experimental data, obtained with in vivo NMR spectroscopy and 13C-labelled substrates, allowed us to describe how the fermentative metabolism in Saccharomyces cerevisiae, taken as eukaryotic cell model, is influenced by stress factors. Experiments on cellular cultures subject to increasing concentrations of ferric ions were conducted in order to study the effect of oxidative stress on the dynamics of the fermentative process. The developed mathematical model was able to simulate the cellular activity, the metabolic yield and the main metabolic fluxes occurring during fermentation and to describe how these are modulated by the presence of ferric ions.
运用体内 NMR 光谱和 13C 标记底物获得的实验数据进行数学建模分析，使我们能够描述酵母（酿酒酵母）发酵代谢受到应激因素的影响。通过对暴露于递增浓度三价铁离子的细胞培养物进行实验，研究了氧化应激对发酵过程动态的影响。所开发的数学模型能够模拟细胞活力、代谢产物和发酵过程中发生的主要代谢通量，并描述三价铁离子的存在如何调节这些参数。
