Centre for Biochemistry and Molecular Cell Biology, Göttingen University, Göttingen, Germany.
RNA Biol. 2013 Jan;10(1):4-18. doi: 10.4161/rna.21879. Epub 2012 Aug 24.
Ribosome synthesis requires a multitude of cofactors, among them DExD/H-box RNA helicases. Bacterial RNA helicases involved in ribosome assembly are not essential, while eukaryotes strictly require multiple DExD/H-box proteins that are involved in the much more complex ribosome biogenesis pathway. Here, RNA helicases are thought to act in structural remodeling of the RNPs including the modulation of protein binding, and they are required for allowing access or the release of specific snoRNPs from pre-ribosomes. Interestingly, helicase action is modulated by specific cofactors that can regulate recruitment and enzymatic activity. This review summarizes the current knowledge and focuses on recent findings and open questions on RNA helicase function and regulation in ribosome synthesis.
核糖体合成需要多种辅助因子，其中包括 DExD/H 盒 RNA 解旋酶。参与核糖体组装的细菌 RNA 解旋酶不是必需的，而真核生物则严格需要多种 DExD/H 盒蛋白，这些蛋白参与更复杂的核糖体生物发生途径。在这里，RNA 解旋酶被认为作用于包括调节蛋白结合在内的 RNP 的结构重塑，并且它们对于允许特定 snoRNP 从前核糖体上进入或释放是必需的。有趣的是，解旋酶的作用受到特定辅助因子的调节，这些辅助因子可以调节募集和酶活性。本综述总结了当前的知识，并重点介绍了 RNA 解旋酶在核糖体合成中的功能和调节的最新发现和未解决的问题。
