Sajid Muhammad, Ahmad Mansur-ud-Din, Khan Muhammad Athar, Anjum Muhammad Aftab, Mushtaq Muhammad Hassan
Department of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Trop Anim Health Prod. 2013 Feb;45(2):693-4. doi: 10.1007/s11250-012-0247-5. Epub 2012 Aug 26.
The present study was an attempt to elucidate the seroprevalence of equine influenza virus (H3N8) in two geographically distinct regions of Pakistan where vaccination is not routinely practiced.
A total number of 315 animals of family Equidae were included in the survey. Blood samples and nasal swabs were collected from the same animal and analyzed through ELISA and Hemagglutination Inhibition.
The seroprevalence for EIV was 10 and 8.39 in districts under study. Out of 29 ELISA positive sera, 7 (24.1 %) showed antibodies against H1 and 22 (75.9 %) showed against H3 of influenza strains. Specie-wise antibody titer against H1 was 7.5 and 9.0 for horses and donkeys, respectively, while mules were negative. Similarly, antibody titer against H3 was 7.12, 6.9, and 6.0 for horses, donkeys, and mules, respectively. No strain was isolated from the nasal swabs despite very high antibody titers of H1 and H3 in the sera of the same animals.
Influenza viruses are circulating in equine species and continuous surveillance is needed to keep check on any future outbreak.
本研究旨在阐明在巴基斯坦两个地理上不同且未常规进行疫苗接种的地区马流感病毒（H3N8）的血清流行率。
调查共纳入315匹马科动物。从同一动物采集血样和鼻拭子，并通过酶联免疫吸附测定（ELISA）和血凝抑制试验进行分析。
在研究的地区，马流感病毒的血清流行率分别为10%和8.39%。在29份ELISA阳性血清中，7份（24.1%）显示出针对流感毒株H1的抗体，22份（75.9%）显示出针对H3的抗体。按物种划分，马和驴针对H1的抗体效价分别为7.5和9.0，而骡子为阴性。同样，马、驴和骡子针对H3的抗体效价分别为7.12、6.9和6.0。尽管同一动物血清中H1和H3的抗体效价很高，但鼻拭子中未分离出毒株。
流感病毒在马科动物中传播，需要持续监测以控制未来的任何疫情爆发。
