Loh Y P, Zucker L, Verspaget H, Van Wimersma Greidanus T B
J Neurosci Res. 1979;4(2):147-56. doi: 10.1002/jnr.490040208.
A major and several minor trichloroacetic acid (TCA) soluble, bioassayable melanotropic peptides, as well as bioreactive and immunoreactive alpha-MSH have been found in the hypothalamus, olfactory bulb and cerebral cortex of normal and hypophysectomized rats. By employing subcellular fractionation procedures it was demonstrated that alpha-MSH and the major TCA soluble melanotropic peptide (MMPB) were localized in synaptosomes and were released by hypoosmotic shock. The analysis of MMPB by electrophoretic and chromatographic procedures reveales that it is not ACTH4-10, ACTH1-10, ACTH1-24, NAcACTH1-10 or alpha-MSH. MMPB was found to cross-react with an antiserum specific for the Lys-Pro-Val NH2 sequence in alpha-MSH, indicating that this C-terminal sequence of alpha-MSH may be present in its structure. MMPB was also shown to differ electrophoretically from the two major TCA soluble melanotropic peptides found in the neurointermediate lobe of the pituitary. In view of their synaptosomal localizations MMPB and alpha-MSH may play a role in synaptic function in the nervous system.
在正常大鼠和垂体切除大鼠的下丘脑、嗅球和大脑皮层中发现了一种主要的和几种次要的可溶于三氯乙酸（TCA）、具有生物活性的促黑素肽，以及具有生物反应性和免疫反应性的α-促黑素（α-MSH）。通过采用亚细胞分级分离程序证明，α-MSH和主要的可溶于TCA的促黑素肽（MMPB）定位于突触体中，并通过低渗休克释放。通过电泳和色谱程序对MMPB的分析表明，它不是促肾上腺皮质激素4-10、促肾上腺皮质激素1-10、促肾上腺皮质激素1-24、N-乙酰促肾上腺皮质激素1-10或α-MSH。发现MMPB与针对α-MSH中Lys-Pro-Val NH2序列的抗血清发生交叉反应，表明α-MSH的这种C末端序列可能存在于其结构中。MMPB在电泳上也与在垂体神经中间叶中发现的两种主要的可溶于TCA的促黑素肽不同。鉴于它们在突触体中的定位，MMPB和α-MSH可能在神经系统的突触功能中发挥作用。
