Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
J Virol. 2012 Sep;86(18):10234-5. doi: 10.1128/JVI.01579-12.
φCbK is a B3 morphotype bacteriophage of the Siphoviridae family that infects Caulobacter crescentus, the preeminent model system for bacterial cell cycle studies. The last 4 decades of research with φCbK as a genetic and cytological tool to study the biology of the host warrant an investigation of the phage genome composition. Herein, we report the complete genome sequence of φCbK and highlight unusual features that emerged from its annotation. The complete genome analysis of the φCbK phage provides new insight into its characteristics and potential interactions with its Caulobacter crescentus host, setting the stage for future functional studies with φCbK.
φCbK 是一种属于长尾噬菌体科的 B3 形态噬菌体,可感染新月柄杆菌,该菌是研究细菌细胞周期的主要模式系统。过去 40 年的研究将 φCbK 作为一种遗传和细胞学工具,用于研究宿主的生物学,这证明了对噬菌体基因组组成进行调查是合理的。在此,我们报告了 φCbK 噬菌体的完整基因组序列,并强调了其注释中出现的不寻常特征。对 φCbK 噬菌体的完整基因组分析为其特性及其与新月柄杆菌宿主的潜在相互作用提供了新的认识,为未来使用 φCbK 进行功能研究奠定了基础。