Fisher P B, Weinstein I B, Eisenberg D, Ginsberg H S
Proc Natl Acad Sci U S A. 1978 May;75(5):2311-4. doi: 10.1073/pnas.75.5.2311.
The tumor-promoting agent 12-O-tetradecanoyl-phorbol-13-acetate (TPA) caused a 2- to 3-fold enhancement of transformation of secondary rat embryo cells that had been injected with a temperature-sensitive mutant of adenovirus type 5(H5ts 125). In addition, transformed foci appeared earlier and were larger in cultures grown in the presence of TPA. The addition of TPA could be delayed until up to 7 days after viral injection and still enhancement was observed. Exposure of the cells to 7,12-dimethylbenz[a]pyrene prior to H5ts125 infection also resulted in a 2- to 4-fold enhancement of transformation, and this enhancement was further augmented 2- to 3-fold when cells were grown in TPA after virus infection. Whereas TPA did not enhance the cloning efficiency of normal rat embryo cells, it did enhance the cloning efficiency of isolated colonies of adenovirus-transformed cells when these were grown alone or cocultured with a 100-fold excess of normal rat embryo cells. The enhancement of adenovirus transformation by TPA appears to be due to its ability to facilitate expression of the transformed state rather than an effect on virus uptake or integration.
肿瘤促进剂12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）使注射了5型腺病毒温度敏感突变体（H5ts 125）的二代大鼠胚胎细胞的转化增强了2至3倍。此外，在TPA存在下培养的细胞中，转化灶出现得更早且更大。TPA的添加可以延迟到病毒注射后7天，仍然能观察到增强作用。在H5ts125感染之前，将细胞暴露于7,12 - 二甲基苯并[a]芘也导致转化增强了2至4倍，并且当病毒感染后细胞在TPA中生长时，这种增强作用进一步增强了2至3倍。虽然TPA没有提高正常大鼠胚胎细胞的克隆效率，但当单独培养或与100倍过量的正常大鼠胚胎细胞共培养时，它确实提高了腺病毒转化细胞分离克隆的克隆效率。TPA对腺病毒转化的增强作用似乎是由于它能够促进转化状态的表达，而不是对病毒摄取或整合的影响。
