Department of Physiology, Immunology Laboratory, University of Calcutta, University Colleges of Science and Technology, Calcutta, West Bengal, India.
Scand J Immunol. 2012 Dec;76(6):528-40. doi: 10.1111/j.1365-3083.2012.02766.x.
To study the effects of gentamicin in combination with ascorbic acid on septic arthritis, mice were infected with Staphylococcus aureus (S. aureus) and treated with gentamicin, which was given at 5 mg/kg after 24 h of infection, followed by ascorbic acid, given at 20 mg/kg body weight after 2 h of gentamicin treatment. Mice were sacrificed at 3, 9, 15 days post-infection (dpi). Combined treatment of infected mice with gentamicin and ascorbic acid eradicated the bacteria from the blood, spleen and synovial tissue and showed a significant gross reduction in arthritis, reduced serum levels of tumour necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ). S. aureus-infected mice have demonstrated the disturbed antioxidant status measured in terms of cellular antioxidants like reduced glutathione and antioxidant enzymes such as superoxide dismutase (SOD) and catalase. The same were ameliorated when the animals were co-treated with gentamicin along with ascorbic acid.
为了研究庆大霉素与抗坏血酸联合应用对脓毒性关节炎的影响，将金黄色葡萄球菌（金黄色葡萄球菌）感染的小鼠用庆大霉素治疗，在感染后 24 小时给予 5mg/kg，然后在庆大霉素治疗后 2 小时给予 20mg/kg 体重的抗坏血酸。在感染后 3、9、15 天处死小鼠。感染小鼠联合应用庆大霉素和抗坏血酸可从血液、脾脏和滑膜组织中清除细菌，并显著减轻关节炎的严重程度，降低血清肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）水平。金黄色葡萄球菌感染的小鼠表现出抗氧化状态紊乱，可通过细胞抗氧化剂如还原型谷胱甘肽和抗氧化酶如超氧化物歧化酶（SOD）和过氧化氢酶来衡量。当动物与庆大霉素联合使用抗坏血酸时，这些情况得到改善。
