Mal Pinky, Ghosh Deboshree, Bandyopadhyay Debasish, Dutta Kallol, Bishayi Biswadev
Department of Physiology, Immunology Laboratory, University Colleges of Science and Technology, University of Calcutta, 92 APC Road, Kolkata 700 009, India.
Indian J Exp Biol. 2012 Oct;50(10):677-89.
Effects of ampicillin (Amp) in combination with riboflavin on septic arthritis in mice infected with Staphylococcus aureus have been reported. Ampicillin was given at 100 mg/kg after 24 h of infection, followed by riboflavin (Ribo) at 20 mg/kg body wt, after 2 h of Amp treatment. Mice were sacrificed at 3, 9, 15 days post infection (dpi). Combined treatment of infected mice with ampicillin and riboflavin eradicated the bacteria from blood, spleen and synovial tissue and showed a significant gross reduction in arthritis, reduced serum levels of TNF-alpha and IFN-gamma. S. aureus infected mice exhibited higher synovial TNF-alpha and IL-6, which was also reduced by ampicillin and riboflavin treatment. S. aureus infected mice showed a disturbed antioxidant status measured in terms of cellular anti-oxidants like reduced glutathione and anti-oxidant enzymes such as superoxide dismutase and catalase and were ameliorated when the animals were co-treated with ampicillin along with riboflavin. Results of the study showed that combined treatment with anti-oxidant and antibiotic may protect from staphylococcal arthritis and may ameliorate oxidative stress caused by S. aureus infection.
已有报道氨苄西林（Amp）与核黄素联合使用对感染金黄色葡萄球菌的小鼠脓毒性关节炎的影响。感染24小时后给予100mg/kg氨苄西林，氨苄西林治疗2小时后，再给予20mg/kg体重的核黄素（Ribo）。在感染后3、9、15天处死小鼠。用氨苄西林和核黄素联合治疗感染小鼠，可清除血液、脾脏和滑膜组织中的细菌，并显著减轻关节炎的总体症状，降低血清中肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）的水平。感染金黄色葡萄球菌的小鼠滑膜TNF-α和白细胞介素-6（IL-6）水平较高，氨苄西林和核黄素治疗也可使其降低。感染金黄色葡萄球菌的小鼠在细胞抗氧化剂如还原型谷胱甘肽以及超氧化物歧化酶和过氧化氢酶等抗氧化酶方面的抗氧化状态受到干扰，当动物同时用氨苄西林和核黄素联合治疗时得到改善。研究结果表明，抗氧化剂和抗生素联合治疗可能预防葡萄球菌性关节炎，并改善由金黄色葡萄球菌感染引起的氧化应激。
