Alvarez G M, Dalvit G C, Cetica P D
Institute of Research and Technology in Animal Reproduction, Area of Biochemistry, School of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina.
Reprod Domest Anim. 2012 Oct;47(5):856-64. doi: 10.1111/j.1439-0531.2011.01943.x.
The aim of this work was to examine the influence of the cumulus and gonadotropins on the metabolic profile of porcine cumulus oocyte complexes (COCs) during in vitro maturation. Immature COCs were assigned to morphological classes A(1) (with a dense cumulus), A(2) (with a translucent cumulus), B(1) (with the corona radiata), B(2) (with only some remaining cumulus cells) and matured with or without gonadotropins. Glycolysis and ammonia production were higher in the A class COCs; gonadotropins increased both, especially in the A(1) COCs (p < 0.05). The A class COCs had the highest initial protein contents and at the end of in vitro maturation. Furthermore, hormonal stimulation induced a similar increase in protein contents of both A classes (p < 0.05). The neutral lipid content and reactive oxygen species (ROS) levels were similar in the immature oocytes of the COCs of all classes. A reduction was seen in both these variables when maturation proceeded either in the presence or absence of gonadotropins. The cumulus type surrounding the oocyte is related to the metabolism of carbohydrates and amino acids by the COC during in vitro maturation under gonadotropic stimulation. Oocyte lipolytic activity and ROS production appear to be independent of the surrounding cumulus and the presence of gonadotropins.
本研究的目的是检测卵丘和促性腺激素对猪卵丘卵母细胞复合体（COCs）体外成熟过程中代谢概况的影响。未成熟的COCs被分为形态学A(1)类（卵丘致密）、A(2)类（卵丘半透明）、B(1)类（有放射冠）、B(2)类（仅残留一些卵丘细胞），并在有或无促性腺激素的情况下进行成熟培养。A类COCs的糖酵解和氨生成较高；促性腺激素使二者均增加，尤其是A(1)类COCs（p<0.05）。A类COCs在体外成熟开始时和结束时蛋白质含量最高。此外，激素刺激使两类A类COCs的蛋白质含量均有类似增加（p<0.05）。所有类别的COCs未成熟卵母细胞中的中性脂质含量和活性氧（ROS）水平相似。无论有无促性腺激素，随着成熟过程的进行，这两个变量均降低。在促性腺激素刺激下，卵母细胞周围卵丘的类型与COCs在体外成熟过程中碳水化合物和氨基酸代谢有关。卵母细胞的脂解活性和ROS生成似乎与周围卵丘及促性腺激素的存在无关。
