Department of Otorhinolaryngology, Medical University of Vienna, Vienna, Austria.
Clin Exp Allergy. 2012 Sep;42(9):1347-55. doi: 10.1111/j.1365-2222.2012.04030.x.
The production of allergen-specific IgE antibodies is a hallmark of IgE-mediated allergy but the contribution of blood cells to allergen-specific IgE production in allergic patients has not been studied in detail.
Aim of this study was the characterization of IgE-producing cells in the blood of allergic patients and the determination of the amount of IgE antibodies which are produced by these cells in relation to total amounts of circulating specific IgE.
Peripheral blood mononuclear cells (PBMCs) were isolated from allergic patients and cell populations were purified or depleted using magnetically labelled antibodies directed against specific cell surface markers (CD19, CD20, CD22, CD27, CD38, CD126, CD138, CD203c). Allergen-specific IgE was measured in serum samples and cell culture supernatants by quantitative ImmunoCAP measurements and by ELISA using purified recombinant allergens. IgE transcripts were detected using RT-PCR with primers specific for human IgE.
We found that allergen-specific IgE levels in PBMC supernatants correlated strongly with specific serum IgE but represented less than 1% of circulating IgE. Depletion of basophils resulted in substantial reduction of allergen-specific IgE levels in PBMC culture supernatants suggesting that an important source of allergen-specific IgE in PBMC supernatants could be IgE derived from the surface of basophils. Newly synthesized IgE was derived from CD138+ plasma cells, but not from B and B memory cells, and accounted for only approximately 0.2% of circulating IgE in blood.
Our finding that the majority of allergen-specific IgE in the peripheral blood is not derived from IgE-secreting cells in the blood suggests local IgE production in tissues as a major source for allergen-specific IgE and possible target for therapeutic intervention.
过敏原特异性 IgE 抗体的产生是 IgE 介导过敏的标志，但血液细胞在过敏性患者中产生过敏原特异性 IgE 的贡献尚未详细研究。
本研究的目的是表征过敏性患者血液中的 IgE 产生细胞，并确定这些细胞产生的 IgE 抗体量与循环特异性 IgE 的总量之间的关系。
从过敏性患者中分离外周血单核细胞（PBMC），并使用针对特定细胞表面标志物（CD19、CD20、CD22、CD27、CD38、CD126、CD138、CD203c）的磁性标记抗体对细胞群体进行纯化或耗尽。使用定量 ImmunoCAP 测量法和使用纯化的重组过敏原的 ELISA 在血清样本和细胞培养上清液中测量过敏原特异性 IgE。使用针对人 IgE 的引物通过 RT-PCR 检测 IgE 转录物。
我们发现 PBMC 上清液中的过敏原特异性 IgE 水平与特异性血清 IgE 强烈相关，但仅占循环 IgE 的不到 1%。嗜碱性粒细胞的耗竭导致 PBMC 培养上清液中过敏原特异性 IgE 水平的显著降低，这表明 PBMC 上清液中过敏原特异性 IgE 的一个重要来源可能是源自嗜碱性粒细胞表面的 IgE。新合成的 IgE 来自 CD138+浆细胞，但不是 B 和 B 记忆细胞，仅占血液中循环 IgE 的约 0.2%。
我们的发现表明，外周血中的大多数过敏原特异性 IgE 不是来自血液中的 IgE 分泌细胞，这表明组织中的局部 IgE 产生是过敏原特异性 IgE 的主要来源，可能是治疗干预的目标。
Zotero 插件