Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States.
Front Immunol. 2023 Feb 2;14:1104609. doi: 10.3389/fimmu.2023.1104609. eCollection 2023.
IgE+ plasmablasts develop following allergen exposure and B cell activation. They secrete IgE and therefore are directly linked to maintain the mechanisms of IgE-mediated allergies. Here, we show that the presence of IgE+ plasmablasts in peripheral blood not only coincides with clinical allergy, but also predicts the upcoming development of clinical disease.
Using an equine model of naturally occurring allergy, we compared the timing of allergen exposure, arrival of IgE+ plasmablasts in peripheral blood, and onset of clinical disease.
We found that IgE+ plasmablasts predict the development of clinical allergy by at least 3 weeks and can be measured directly by flow cytometry or by IgE secretion following culture. We also compared the IgE secretion by IgE+ plasmablasts with total plasma IgE concentrations and found that while IgE secretion consistently correlates with clinical allergy, total plasma IgE does not.
Together, we describe IgE+ plasmablasts as a reliable and sensitive predictive biomarker of allergic disease development.
IgE+浆母细胞在过敏原暴露和 B 细胞激活后发展。它们分泌 IgE，因此与 IgE 介导的过敏的维持机制直接相关。在这里，我们表明外周血中 IgE+浆母细胞的存在不仅与临床过敏相符，而且还预测了即将发生的临床疾病。
使用自然发生过敏的马模型，我们比较了过敏原暴露的时间、IgE+浆母细胞在外周血中的出现时间以及临床疾病的发病时间。
我们发现 IgE+浆母细胞至少可以提前 3 周预测临床过敏的发生，并且可以通过流式细胞术或培养后的 IgE 分泌直接测量。我们还比较了 IgE+浆母细胞的 IgE 分泌与总血浆 IgE 浓度，发现尽管 IgE 分泌与临床过敏一致，但总血浆 IgE 则不然。
总之，我们将 IgE+浆母细胞描述为过敏疾病发展的可靠且敏感的预测生物标志物。
