Miki K, Ezoe T, Masui A, Yoshisaka T, Mimuro M, Fujiwara-Arasaki T, Kasai N
Department of Applied Chemistry, Faculty of Engineering, Osaka University.
J Biochem. 1990 Oct;108(4):646-9. doi: 10.1093/oxfordjournals.jbchem.a123256.
C-Phycocyanin from a red alga, Porphyra tenera, has been crystallized by the vapor-diffusion procedure. Both orthorhombic and hexagonal forms were obtained from ammonium sulfate solutions, whereas only the orthohombic form was selectively grown from sodium citrate solutions. The orthorhombic crystals are more suitable for further crystallographic work; their space group is P2(1)2(1)2(1), with unit-cell dimensions of a = 105, b = 121, and c = 184 A. The asymmetric unit comprises two (alpha beta)3 trimer molecules of C-phycocyanin. These crystals diffract X-rays up to about 3 A resolution.
