Kaszuba M, Hunt G R
Department of Science and Chemical Engineering, Polytechnic of Wales, Pontypridd, Mid-Glamorgan.
J Inorg Biochem. 1990 Nov;40(3):217-25. doi: 10.1016/0162-0134(90)80055-3.
1H-NMR spectroscopy of small, unilamellar dipalmitoyl phosphatidylcholine (DPPC) vesicles in conjunction with the lanthanide shift reagent Pr3+ was used to study the effect of Zn2+ and Ca2+ ions on the permeability induced at the lipid phase transition temperature (Tc) of the vesicles and by the bee venom polypeptide melittin. In addition, the effects of Zn2+ and Ca2+ were studied on Triton X-100 stabilized channels at Tc and in the presence of n-alcohols. The results show that the presence of 10 mM Zn2+ and Ca2+ inhibited most of the forms of vesicular permeability investigated. The results are discussed in terms of the nature of the binding of the metal ions to the vesicles and support the proposal that one biological function of Zn2+ and Ca2+ is protection against membrane damage.
利用小的、单层二棕榈酰磷脂酰胆碱（DPPC）囊泡的1H-NMR光谱结合镧系位移试剂Pr3+，研究了Zn2+和Ca2+离子对囊泡在脂质相变温度（Tc）时以及被蜂毒多肽蜂毒素诱导的通透性的影响。此外，还研究了Zn2+和Ca2+在Tc以及存在正醇的情况下对Triton X-100稳定通道的影响。结果表明，10 mM Zn2+和Ca2+的存在抑制了所研究的大多数囊泡通透性形式。根据金属离子与囊泡结合的性质对结果进行了讨论，并支持了Zn2+和Ca2+的一种生物学功能是防止膜损伤的观点。
