Hunt G R, Kaszuba M
Department of Science, Polytechnic of Wales, Pontypridd, Mid Glamorgan, U.K.
Chem Phys Lipids. 1989 Jul;51(1):55-65. doi: 10.1016/0009-3084(89)90066-2.
The effect of a series of n-alcohols on the permeability of small, unilamellar dipalmitoyl phosphatidylcholine (DPPC), dimyristoyl phosphatidylcholine (DMPC) and distearoyl phosphatidylcholine (DSPC) vesicles at the gel-to-liquid crystal phase transition temperature was investigated. It was found that the permeability took the form of the transient lysis of a fraction of the population of vesicles. The effect on this lysis of the n-alcohols was seen to be very chain-length dependent, with a minimum at n = 8 (octan-1-ol) for DPPC vesicles. A similar minimum was observed in the presence of 0.1 mM Triton X-100, but the detergent could then interact with certain of the alcohols to produce permanent channels. The results are discussed in terms of the semi-empirical model of Brasseur et al. (1985) Biochim. Biophys. Acta 814, 227-236, for the interaction of the n-alcohols with a DPPC membrane. The effect of various n-alcohols on the outer and inner monolayers of DPPC vesicles was also studied and the results related to their fluidising effect, allowing channels to open at the phase transition temperature.
研究了一系列正醇在凝胶态向液晶态转变温度下对小的、单层二棕榈酰磷脂酰胆碱（DPPC）、二肉豆蔻酰磷脂酰胆碱（DMPC）和二硬脂酰磷脂酰胆碱（DSPC）囊泡通透性的影响。发现通透性表现为一部分囊泡群体的瞬时裂解形式。观察到正醇对这种裂解的影响非常依赖链长，对于DPPC囊泡，在n = 8（1-辛醇）时最小。在存在0.1 mM Triton X-100的情况下观察到类似的最小值，但去污剂随后可与某些醇相互作用产生永久性通道。根据Brasseur等人（1985年，《生物化学与生物物理学报》814卷，227 - 236页）关于正醇与DPPC膜相互作用的半经验模型对结果进行了讨论。还研究了各种正醇对DPPC囊泡外层和内层单分子层的影响，并将结果与其流化作用相关联，使通道在相变温度下打开。
