Zheng He-zhong, Xu Ke-wen, Yi Hong, Dong Wei, Wang Yu-lan, Ni Qi, Weng Jing
Department of Neurology, The People's Hospital of Yuyao City, Yuyao 315400, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2012 Jul;41(4):425-9. doi: 10.3785/j.issn.1008-9292.2012.04.011.
To investigate the effect of Colquhounia root tablet on IL-2 and IFN-γ mRNA expression in experimental allergic encephalomyelitis (EAE) of rats.
The allergic encephalomyelitis model was established in Wistar rats by immunization with myelin basic protein of spinal cord of guinea pig and complete Freund's adjuvant. The rats in treatment group received Colquhounia root tablet (300 mg*kg(-1), BID). The symptom of EAE was observed; pathological feature and myelin of brain and spinal cord were detected with HE stain and Loyez's stain, respectively. The expressions of IL-2 and IFN-γ mRNA were assayed by RT-PCR.
No EAE symptoms were developed in treatment group, the expressions of IL-2 and IFN-γ mRNA were 0.345 ± 0.032 and 0.353 ± 0.023, which were significantly lower than those of model group (P<0.01). The histopathologic examinations revealed that less inflammation cells around vessels and demyelination in white matter of brain and spinal cords were observed in treatment group than in model group.
Colquhounia root tablets are effective in treatment of EAE of rats, which may be associated with inhibition of the expression of IL-2 and IFN-γ mRNA.
探讨火把花根片对大鼠实验性变态反应性脑脊髓炎（EAE）中白细胞介素-2（IL-2）和干扰素-γ（IFN-γ）mRNA表达的影响。
采用豚鼠脊髓髓鞘碱性蛋白与完全弗氏佐剂免疫Wistar大鼠，建立变态反应性脑脊髓炎模型。治疗组大鼠给予火把花根片（300mg·kg⁻¹，每日2次）。观察EAE症状；分别用苏木精-伊红（HE）染色和洛耶兹（Loyez）染色检测脑和脊髓的病理特征及髓鞘。采用逆转录-聚合酶链反应（RT-PCR）检测IL-2和IFN-γ mRNA的表达。
治疗组未出现EAE症状，IL-2和IFN-γ mRNA表达分别为0.345±0.032和0.353±0.023，显著低于模型组（P<0.01）。组织病理学检查显示，治疗组脑和脊髓白质血管周围炎症细胞较少，脱髓鞘程度较轻。
火把花根片对大鼠EAE有治疗作用，可能与抑制IL-2和IFN-γ mRNA表达有关。
