Unitat de Farmacologia i Farmacognòsia, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain.
Phytother Res. 2013 Jun;27(6):911-5. doi: 10.1002/ptr.4806. Epub 2012 Aug 23.
The antifungal activity of hexane, dichloromethane, methanol and aqueous extracts from the rhizome and root of Ferula hermonis was assayed in vitro by the agar disk diffusion method against a panel of human opportunistic and pathogenic fungi. Among them, the hexane and dichloromethane extracts showed the highest activity particularly against the dermatophytes Microsporum gypseum and Tricophyton mentagrophytes as well as the yeast Candida lactis-condensi. Activity-guided fractionation of both extracts using an agar overlay bioautographic method led to the isolation of two antifungal compounds which were identified as the daucane aryl esters jaeschkeanadiol p-hydroxybenzoate (ferutinin) and jaeschkeanadiol benzoate (teferidin). Determination of minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) values of both compounds evidenced a stronger antifungal activity for ferutinin than for teferidin. Particularly, T. mentagrophytes was the most sensitive strain with MIC and MFC values ranging from 8 to 256 µg/mL.
采用琼脂圆盘扩散法，测定了新疆阿魏根和根茎的正己烷、二氯甲烷、甲醇和水提取物对一组人体机会致病性真菌的体外抗真菌活性。其中，正己烷和二氯甲烷提取物对皮肤真菌石膏样小孢子菌和须癣毛癣菌以及酵母假丝酵母-condensi 表现出最高的活性。采用琼脂覆盖生物自显影法对两种提取物进行活性导向分离，得到了两种具有抗真菌活性的化合物，鉴定为大根香叶基芳基酯 jaeschkeanadiol p-羟基苯甲酸酯（ferutinin）和 jaeschkeanadiol 苯甲酸酯（teferidin）。两种化合物的最小抑菌浓度（MIC）和最小杀菌浓度（MFC）值的测定表明，ferutinin 的抗真菌活性强于 teferidin。特别是 T. mentagrophytes 是最敏感的菌株，MIC 和 MFC 值范围为 8 至 256µg/mL。
