贺蒙阿魏根和根茎精油的组成与抗真菌活性。

Composition and antifungal activity of the essential oil from the rhizome and roots of Ferula hermonis.

机构信息

Unitat de Farmacologia i Farmacognòsia, Facultat de Farmàcia, Universitat de Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain.

出版信息

Phytochemistry. 2011 Aug;72(11-12):1406-13. doi: 10.1016/j.phytochem.2011.04.013. Epub 2011 May 24.

DOI:10.1016/j.phytochem.2011.04.013

PMID:21609848

Abstract

The analysis of the essential oil from rhizome and roots of Ferula hermonis Boiss. (Apiaceae) by GC-FID, GC-MS and ¹³C NMR allowed the identification of 79 constituents, more than 90% of the oil, the major one being α-pinene (43.3%), followed by α-bisabolol (11.1%) and the unusual acetylenic compound 3,5-nonadiyne (4.4%). The antifungal activity of the essential oil before and after fractionation was assayed against several yeasts and filamentous fungi. Purification of the active fractions afforded 3,5-nonadiyne, α-bisabolol, jaeschkeanadiol angelate, α-bisabolol oxide B and trans-verbenol, as well as two purified fractions, one of them (JB73) with 73% of jaeschkeanadiol benzoate and the other with 50% of spathulenol. Determination of MIC and MFC values of all these products evidenced strong antifungal activities for JB73 and 3,5-nonadiyne. Particularly, against the dermatophyte Tricophyton mentagrophytes, MIC and MFC values were 0.25 μg/ml for JB73, and 8 μg/ml for 3,5-nonadiyne, the former being more active than amphotericin B and nystatin.

摘要

通过 GC-FID、GC-MS 和 ¹³C NMR 对 Ferula hermonis Boiss.（伞形科）的根茎精油进行分析，鉴定出 79 种成分，超过 90%的精油，其中主要成分是α-蒎烯（43.3%），其次是α-倍半萜醇（11.1%）和不常见的炔属化合物 3,5-壬二炔（4.4%）。对精油进行分离前后的抗真菌活性进行了测试，结果表明对几种酵母和丝状真菌都有活性。对活性馏分进行纯化，得到 3,5-壬二炔、α-倍半萜醇、jaeschkeanadiol 苯甲酸酯、α-倍半萜醇氧化物 B 和反马鞭烯醇，以及两个纯化馏分，其中一个（JB73）含有 73%的 jaeschkeanadiol 苯甲酸酯，另一个含有 50%的 spathulenol。所有这些产物的 MIC 和 MFC 值的测定表明 JB73 和 3,5-壬二炔具有很强的抗真菌活性。特别是对皮肤真菌须癣毛癣菌，JB73 的 MIC 和 MFC 值分别为 0.25 μg/ml 和 3,5-壬二炔 8 μg/ml，前者比两性霉素 B 和制霉菌素更有效。