Suzuki E, Kurata T, Arakawa N
Department of Food and Nutrition, Ochanomizu University, Tokyo, Japan.
J Nutr Sci Vitaminol (Tokyo). 1990 Jun;36(3):235-41. doi: 10.3177/jnsv.36.235.
The enzyme activities, which are influenced by the vitamin C level in tissues, were measured to evaluate the vitamin C activity of erythorbic acid (ErA) in guinea pigs administered ErA. Guinea pigs were divided into two groups: animals in one group (control group) were administered 1, 5, and 100 mg/day ascorbic acid (AsA) and those in the other group (supplemented group) were administered 1, 5, 20, and 100 mg/day ErA for 16 days. At the end of the experimental period, they were sacrificed, blood was collected, and their livers were removed. The activities of liver aniline hydroxylase, of liver acid phosphatase, and of serum alkaline phosphatase, and the content of liver cytochrome P-450 were assayed. The activities of aniline hydroxylase and serum alkaline phosphatase and the content of liver cytochrome P-450 of the guinea pigs administered 1 mg ErA were lower than those of the guinea pigs administered 1 mg AsA. However, the enzyme activities and liver cytochrome P-450 content in the guinea pigs administered 5 mg or more of ErA were similar in level to those in the guinea pigs administered 5 mg AsA. These results suggested that administration of a considerably high amount of ErA to guinea pigs showed a similar vitamin C activity to that of AsA, which might suggest that vitamin C activity of ErA may be more than one-twentieth that of AsA, as has been generally believed.
测量受组织中维生素C水平影响的酶活性，以评估给予异抗坏血酸（ErA）的豚鼠体内异抗坏血酸的维生素C活性。将豚鼠分为两组：一组动物（对照组）每天给予1、5和100毫克抗坏血酸（AsA），另一组动物（补充组）每天给予1、5、20和100毫克ErA，持续16天。在实验期结束时，将它们处死，采集血液，并取出肝脏。测定肝脏苯胺羟化酶、肝脏酸性磷酸酶和血清碱性磷酸酶的活性，以及肝脏细胞色素P-450的含量。给予1毫克ErA的豚鼠的苯胺羟化酶和血清碱性磷酸酶活性以及肝脏细胞色素P-450的含量低于给予1毫克AsA的豚鼠。然而，给予5毫克或更多ErA的豚鼠的酶活性和肝脏细胞色素P-450含量与给予5毫克AsA的豚鼠的水平相似。这些结果表明，给豚鼠施用相当大量的ErA显示出与AsA相似的维生素C活性，这可能表明ErA的维生素C活性可能比通常认为的AsA的维生素C活性高二十倍以上。
