Georgiou Christiana A, Kapnissi-Christodoulou Constantina P
Department of Chemistry, University of Cyprus, 1 University Avenue, 2109 Aglantzia, Nicosia, Cyprus.
J Chromatogr Sci. 2013 Mar;51(3):286-91. doi: 10.1093/chromsci/bms139. Epub 2012 Aug 27.
This research work describes the development of a fast high-performance liquid chromatography method for the simultaneous determination of five important cholesterol oxidation products (COPs). The influence of various experimental parameters, such as the composition of the mobile phase, the flow rate and the column temperature, were investigated. Baseline separation was achieved by using acetonitrile-methanol-water-isopropanol (67:27:5:1) as a mobile phase with a 10°C column temperature. The developed method demonstrated good linearity and high reproducibility, with relative standard deviation values below 1.26% for all the COPs that were examined. The method was then applied, for the first time, to Cypriot smoked-meat products (lountza and hiromeri). The presence of COPs in these products suggests that the preparation of the meat products, and particularly the smoking process, possibly favors the oxidation of cholesterol. Finally, three different sample preparation procedures were evaluated and the optimum procedure was determined based on recovery, precision and simplicity.
本研究工作描述了一种用于同时测定五种重要胆固醇氧化产物（COPs）的快速高效液相色谱方法的开发。研究了各种实验参数的影响，如流动相组成、流速和柱温。通过使用乙腈 - 甲醇 - 水 - 异丙醇（67:27:5:1）作为流动相，柱温为10°C，实现了基线分离。所开发的方法具有良好的线性和高重现性，对于所有检测的COPs，相对标准偏差值均低于1.26%。该方法首次应用于塞浦路斯烟熏肉制品（lountza和hiromeri）。这些产品中COPs的存在表明，肉制品的制备，特别是烟熏过程，可能有利于胆固醇的氧化。最后，评估了三种不同的样品制备程序，并根据回收率、精密度和简便性确定了最佳程序。
