Yarbrough Jason, Harvey Ralph, Cox Sherry
University of Tennessee, Departments of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, Knoxville, TN 37996, USA.
J Chromatogr Sci. 2012 Mar;50(3):162-6. doi: 10.1093/chromsci/bmr042.
High-performance liquid chromatography method for the determination of propofol has been developed and validated. Following a liquid extraction using ethyl acetate and hexane, samples were separated by reverse-phase high-performance liquid chromatography on an XBridge C(18) column and quantified using fluorescence detection at an excitation of 276 nm and an emission of 310 nm. The mobile phase was a mixture of water (pH 4.0) and acetonitrile, with a flow rate of 1.5 mL/min. The standard curve ranged from 5-2000 ng/mL. Intra- and inter-assay variability for propofol was less than 10%, and the average recovery was greater than 95%. This assay is suitable for use in pharmacokinetic studies.
已开发并验证了用于测定丙泊酚的高效液相色谱法。使用乙酸乙酯和己烷进行液液萃取后，样品在XBridge C(18)柱上通过反相高效液相色谱法进行分离，并在激发波长为276 nm、发射波长为310 nm的条件下使用荧光检测进行定量。流动相为水（pH 4.0）和乙腈的混合物，流速为1.5 mL/min。标准曲线范围为5 - 2000 ng/mL。丙泊酚的批内和批间变异小于10%，平均回收率大于95%。该分析方法适用于药代动力学研究。
