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重组人核心蛋白聚糖通过上调p21抑制肝癌HepG2细胞。

Recombinant human decorin suppresses liver HepG2 carcinoma cells by p21 upregulation.

作者信息

Zhang Yucheng, Wang Yali, Du Zhenwu, Wang Qian, Wu Mei, Wang Xiaofeng, Wang Lingling, Cao Linlin, Hamid Abdu Selim, Zhang Guizhen

机构信息

Central Laboratory, China-Japan Union Hospital, Jilin University, Changchun, People's Republic of China.

出版信息

Onco Targets Ther. 2012;5:143-52. doi: 10.2147/OTT.S32918. Epub 2012 Aug 13.

DOI:10.2147/OTT.S32918
PMID:22927763
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3422087/
Abstract

BACKGROUND

Decorin is a multifunctional molecule of the extracellular matrix and impedes different kinds of tumor cell growth, but the role and molecular mechanism by which decorin inhibits HepG2 cells is not fully understood. Our objective was to construct recombinant human decorin (pcDNA3.1-DCN) and to explore the mechanism by which it inhibits HepG2 cells.

METHODS

This experiment was divided into three groups, ie, a control group, an empty vector group, and a pcDNA3.1-DCN group. pcDNA3.1-DCN was constructed using recombinant DNA technology, and the vector for pcDNA3.1-DCN and pcDNA3.1 was then transfected into HepG2 cells using Lipofectamine 2000.

RESULTS

Compared with cells in the control group and in the empty vector group, growth of cells in the pcDNA3.1-DCN group was significantly suppressed, the ratios of cells in the G0/G1 phases and proportion of early apoptotic cells were significantly increased, and the level of p21(WAF1/CIP1) (p21) protein was markedly upregulated (P < 0.05). However, there was no significant difference among the three groups in p53 protein expression (P > 0.05).

CONCLUSION

The pcDNA3.1-DCN vector was successfully constructed and transfected into HepG2 cells, and decorin overexpression suppressed the growth of HepG2 cells by upregulation of p21 via a p53-independent pathway.

摘要

背景

核心蛋白聚糖是细胞外基质的一种多功能分子，可抑制多种肿瘤细胞的生长，但核心蛋白聚糖抑制肝癌细胞系（HepG2细胞）的作用及分子机制尚未完全明确。我们的目的是构建重组人核心蛋白聚糖（pcDNA3.1-DCN）并探讨其抑制HepG2细胞的机制。

方法

本实验分为三组，即对照组、空载体组和pcDNA3.1-DCN组。采用重组DNA技术构建pcDNA3.1-DCN，然后使用脂质体2000将pcDNA3.1-DCN和pcDNA3.1载体转染至HepG2细胞。

结果

与对照组和空载体组细胞相比，pcDNA3.1-DCN组细胞生长明显受到抑制，G0/G1期细胞比例和早期凋亡细胞比例显著增加，p21（WAF1/CIP1）（p21）蛋白水平明显上调（P<0.05）。然而，三组之间p53蛋白表达无显著差异（P>0.05）。

结论

成功构建pcDNA3.1-DCN载体并将其转染至HepG2细胞，核心蛋白聚糖过表达通过p53非依赖途径上调p21从而抑制HepG2细胞生长。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/3a7964973fce/ott-5-143f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/9ff501d57f75/ott-5-143f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/d53c1a0b3297/ott-5-143f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/1fa783e6e98d/ott-5-143f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/73070610a15d/ott-5-143f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/84893cfa2d70/ott-5-143f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/3a7964973fce/ott-5-143f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/9ff501d57f75/ott-5-143f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/d53c1a0b3297/ott-5-143f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/1fa783e6e98d/ott-5-143f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/73070610a15d/ott-5-143f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/84893cfa2d70/ott-5-143f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5438/3422087/3a7964973fce/ott-5-143f6.jpg

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2
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J Biol Chem. 2011 Oct 7;286(40):34712-21. doi: 10.1074/jbc.M111.262766. Epub 2011 Aug 12.
3
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6
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