西方玉米根萤叶甲（鞘翅目：叶甲科）对 Bt 玉米的适应：遗传、适合度代价和取食偏好。

Adaptation by western corn rootworm (Coleoptera: Chrysomelidae) to Bt maize: inheritance, fitness costs, and feeding preference.

机构信息

Department of Entomology, Iowa State University, Ames, IA 50011, USA.

出版信息

J Econ Entomol. 2012 Aug;105(4):1407-18. doi: 10.1603/ec11425.

Abstract

We examined inheritance of resistance, feeding behavior, and fitness costs for a laboratory-selected strain of western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), with resistance to maize (Zea maize L.) producing the Bacillus thuringiensis Berliner (Bt) toxin Cry3Bb1. The resistant strain developed faster and had increased survival on Bt maize relative to a susceptible strain. Results from reciprocal crosses of the resistant and susceptible strains indicated that inheritance of resistance was nonrecessive. No fitness costs were associated with resistance alleles in the presence of two entomopathogenic nematode species, Steinernema carpocapsae Weiser and Heterorhabditis bacteriophora Poinar. Larval feeding studies indicated that the susceptible and resistant strains did not differ in preference for Bt and non-Bt root tissue in choice assays.

摘要

我们研究了对玉米产生苏云金芽孢杆菌（Bt） Cry3Bb1 毒素的西部玉米根萤叶甲（Diabrotica virgifera virgifera LeConte）（鞘翅目：金龟子科）实验室选择株系的抗性遗传、取食行为和适应代价。与敏感株系相比，抗性株系在 Bt 玉米上的生长速度更快，存活率更高。抗性和敏感株系的正反交结果表明，抗性的遗传是非隐性的。在两种昆虫病原线虫（斯氏线虫 Steinernema carpocapsae Weiser 和霍氏线虫 Heterorhabditis bacteriophora Poinar）存在的情况下，抗性等位基因与适应代价无关。幼虫取食研究表明，在选择试验中，敏感和抗性株系在对 Bt 和非 Bt 根组织的偏好上没有差异。