Department of Pharmacology, School of Medicine, Chonbuk National University, Jeonju, Korea.
Am J Chin Med. 2012;40(5):967-78. doi: 10.1142/S0192415X12500711.
Oxidized low-density lipoprotein (oxLDL) contributes to atherosclerosis in part by being taken up into macrophages via scavenger receptors and leading to foam cell formation. Herbal compounds that have been used to treat blood stasis (a counterpart of atherosclerosis) for centuries include extracts of medicinal plants in the Rosaceae and Leguminosae families. In this study, we investigated the effect of the unripe Rubus coreanus (Korean black raspberry) fruit extract on oxLDL uptake by murine macrophage cells. In the presence of Rubus coreanus extract (RCE), Dil-labeled oxLDL uptake was inhibited in a dose-dependent manner. SP600125, a specific JNK inhibitor, inhibited the uptake of Dil-oxLDL into macrophages. RCE also inhibited JNK phosphorylation in a time- and dose-dependent manner in macrophages treated with oxLDL. These results indicate that among the mitogen-activated protein kinases, JNK phosphorylation is inhibited by RCE, which is likely the mechanism underlying the RCE-induced inhibition of oxLDL uptake by macrophages.
氧化型低密度脂蛋白（oxLDL）通过清道夫受体被巨噬细胞摄取，并导致泡沫细胞形成，从而导致动脉粥样硬化的发生。几个世纪以来，一直有用草药来治疗血瘀（动脉粥样硬化的对应物），包括蔷薇科和豆科药用植物的提取物。在这项研究中，我们研究了未成熟的覆盆子（韩国黑覆盆子）果实提取物对小鼠巨噬细胞摄取 oxLDL 的影响。在覆盆子提取物（RCE）的存在下，Dil 标记的 oxLDL 摄取呈剂量依赖性抑制。SP600125，一种特定的 JNK 抑制剂，抑制了 Dil-oxLDL 进入巨噬细胞的摄取。RCE 还以时间和剂量依赖的方式抑制 oxLDL 处理的巨噬细胞中 JNK 的磷酸化。这些结果表明，在有丝分裂原激活的蛋白激酶中，JNK 磷酸化被 RCE 抑制，这可能是 RCE 诱导的巨噬细胞摄取 oxLDL 抑制的机制。
