Boston, Mass.; Magdeburg, Germany; Padova, Italy; Vienna, Austria; Mainz, Germany; and Santiago, Chile From the Tissue Engineering and Wound Healing Laboratory, Division of Plastic Surgery, and Department of Radiology, Brigham and Women's Hospital and Harvard Medical School; Department of Plastic, Aesthetic, and Hand Surgery, Otto-von-Guericke University of Magdeburg; Institute of Plastic Surgery, University of Padova; Clinical Institute of Pathology, University of Vienna; Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz; Department of Surgery, Pontificia Universidad Católica de Chile; and Back Bay Plastic Surgery and Massachusetts General Hospital-Harvard Medical School.
Plast Reconstr Surg. 2012 Sep;130(3):541-547. doi: 10.1097/PRS.0b013e31825dc04d.
Fat grafting is a powerful tool for soft-tissue reconstruction; however, the science behind recipient bed preparation has not been thoroughly explored. External volume expansion using suction before fat grafting has been used clinically to improve reliability and consistency of graft survival. The authors developed a murine model to investigate the underlying mechanism of external volume expansion.
The authors created an external volume expansion device using a soft-silicone dome connected to a vacuum source (25 mmHg) to treat the dorsum of mice, and the response was compared with treatment with an occlusive dressing. Treated areas were monitored with magnetic resonance imaging. Remodeling of microvasculature was studied with corrosion casting on day 7. Effects on tissue thickness, number of adipocytes, cell proliferation, and blood vessel density were analyzed at 28 days.
Macroscopic analysis showed tissue swelling at sites treated with the external volume expansion device by 21 days, without skin damage. On day 28, external volume expansion increased the thickness of the subcutaneous fat layer twofold, consistent with magnetic resonance imaging observations. The proliferation rate in the subcutaneous layer of expansion-treated areas increased twofold, with a net 2.2-fold increase in number of adipocytes in columns; remodeling of the vessels network occurred, with reorientation and increase of vessel diameters shown by corrosion casting and 1.9-fold augmentation of vessels density.
External volume expansion applied to mouse integument induces highly proliferative and vascularized subcutaneous tissue. Recipient-site preparation using external volume expansion devices may be a promising tool to enhance cell and tissue engraftment.
脂肪移植是软组织重建的有力工具；然而，受体床准备的科学尚未得到彻底探索。在脂肪移植前使用抽吸进行外部容量扩张已在临床上用于提高移植物存活率的可靠性和一致性。作者开发了一种鼠模型来研究外部容量扩张的潜在机制。
作者使用连接到真空源（25mmHg）的软硅胶穹顶创建了一个外部体积扩张装置，以治疗小鼠的背部，并将其与闭塞敷料治疗进行比较。用磁共振成像监测处理区域。在第 7 天用腐蚀铸造研究微血管重塑。在 28 天时分析组织厚度、脂肪细胞数量、细胞增殖和血管密度的影响。
宏观分析显示，通过 21 天的外部体积扩张装置治疗，组织肿胀，没有皮肤损伤。在第 28 天，外部体积扩张将皮下脂肪层的厚度增加了两倍，与磁共振成像观察结果一致。扩张处理区皮下层的增殖率增加了一倍，柱中脂肪细胞数量增加了 2.2 倍；血管网络发生重塑，通过腐蚀铸造显示出血管直径的重新定向和增加，血管密度增加了 1.9 倍。
应用于小鼠体被的外部体积扩张可诱导高度增殖和血管化的皮下组织。使用外部体积扩张装置进行受体部位准备可能是增强细胞和组织移植的有前途的工具。
