Lujan-Hernandez Jorge, Lancerotto Luca, Nabzdyk Christoph, Hassan Kazi Zayn, Giatsidis Giorgio, Khouri Roger K, Chin Michael S, Bassetto Franco, Lalikos Janice F, Orgill Dennis P
Boston and Worcester, Mass.; and Padova, Italy From the Tissue Engineering and Wound Healing Laboratory, Division of Plastic Surgery, Brigham and Women's Hospital-Harvard Medical School; the Division of Plastic Surgery, University of Massachusetts Medical School; and the Clinic of Plastic Surgery, University of Padova.
Plast Reconstr Surg. 2016 Jan;137(1):122-131. doi: 10.1097/PRS.0000000000001859.
External volume expansion by suction is used to prepare the recipient site for fat grafting by increasing its compliance and vascularity. The authors previously developed a mouse model for external volume expansion and demonstrated its pro-proliferative and angiogenic effects. Increased thickness of the subcutaneous tissue was also observed. This study was thus designed to assess the adipogenic potential of external volume expansion stimulation.
A miniaturized external volume expansion device consisting of a rubber dome connected to a -25 mmHg suction source was applied to the dorsum of mice for a single 2-hour stimulation or for 2 hours daily for 5 days. Tissues were harvested up to 48 hours after the last stimulation and analyzed for edema, inflammation, and adipocyte content by staining for hematoxylin and eosin, CD45, and perilipin-A. Expression of peroxisome proliferator-activated receptor-γ (proadipogenic factor) and preadipocyte factor 1 (preadipocyte marker) was evaluated by Western blot analysis.
Both a 2-hour stimulation and cyclical 2-hour stimulation for 5 days induced 1.5- and 1.9-fold increases in the number of adipocytes per millimeter. Edema was present in the immediate poststimulation period, and inflammation was seen 2 days later. Peroxisome proliferator-activated receptor-γ was increased at the end of stimulation.
Stretch is known to stimulate proliferation, whereas edema and inflammation are both emerging proadipogenic factors. Their combination in external volume expansion seems to produce proadipogenic effects, seen even after a single 2-hour stimulation.
通过抽吸进行外部容积扩张可增加受体部位的顺应性和血管生成，从而为脂肪移植准备受体部位。作者之前建立了一种用于外部容积扩张的小鼠模型，并证明了其促增殖和促血管生成作用。还观察到皮下组织厚度增加。因此，本研究旨在评估外部容积扩张刺激的成脂潜力。
将一个由连接到-25 mmHg抽吸源的橡胶圆顶组成的小型外部容积扩张装置应用于小鼠背部，进行单次2小时刺激或每天2小时，持续5天。在最后一次刺激后48小时内收集组织，通过苏木精和伊红染色、CD45染色和脂联素A染色分析水肿、炎症和脂肪细胞含量。通过蛋白质印迹分析评估过氧化物酶体增殖物激活受体γ（促脂肪生成因子）和前脂肪细胞因子1（前脂肪细胞标志物）的表达。
单次2小时刺激和连续5天每天2小时的周期性刺激均使每毫米脂肪细胞数量分别增加了1.5倍和1.9倍。刺激后即刻出现水肿，2天后出现炎症。刺激结束时过氧化物酶体增殖物激活受体γ增加。
已知拉伸可刺激增殖，而水肿和炎症都是新出现的促脂肪生成因子。它们在外部容积扩张中的联合作用似乎产生了促脂肪生成效应，即使在单次2小时刺激后也可见到。
