Brogaard Kristin R, Xi Liqun, Wang Ji-Ping, Widom Jonathan
Department of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA.
Methods Enzymol. 2012;513:315-34. doi: 10.1016/B978-0-12-391938-0.00014-8.
Most eukaryotic DNA exists in DNA-protein complexes known as nucleosomes. The exact locations of nucleosomes along the genome play a critical role in chromosome functions and gene regulation. However, the current methods for nucleosome mapping do not provide the necessary accuracy to identify the precise nucleosome locations. Here we describe a new experimental approach that directly maps nucleosome center locations in vivo genome-wide at single base pair resolution.
大多数真核生物的DNA存在于被称为核小体的DNA-蛋白质复合物中。核小体在基因组上的确切位置在染色体功能和基因调控中起着关键作用。然而，目前用于核小体定位的方法无法提供确定精确核小体位置所需的精度。在此，我们描述了一种新的实验方法，该方法能在全基因组范围内以单碱基对分辨率直接在体内定位核小体中心位置。
