Nowak F V
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-3125.
Mol Endocrinol. 1990 Aug;4(8):1205-10. doi: 10.1210/mend-4-8-1205.
Neuropeptides are central to the regulation of normal sexual development and reproduction. Two new hypothalamic cDNAs have been identified by Northern blot analysis and molecular cloning. Each potentially encodes a precursor for a unique GnRH-like decapeptide. Northern blot analysis reveals tissue-specific transcripts from each gene in the hypothalamus and testis. These preoptic area regulatory factor genes, PORF-1 and PORF-2, may thus encode members of a family of GnRH-related peptides which have been described in these tissues.
神经肽对于正常性发育和生殖的调节至关重要。通过Northern印迹分析和分子克隆鉴定出了两个新的下丘脑cDNA。每个都可能编码一种独特的促性腺激素释放激素（GnRH）样十肽的前体。Northern印迹分析揭示了下丘脑和睾丸中每个基因的组织特异性转录本。因此，这些视前区调节因子基因PORF - 1和PORF - 2可能编码在这些组织中已被描述的GnRH相关肽家族的成员。
