Pfaff D W
J Neurosci Res. 1986;16(1):109-15. doi: 10.1002/jnr.490160111.
In situ hybridization allows the detection and measurement of specific messenger RNAs in individual hypothalamic neurons, and has shown, among magnocellular neurons, not only which cells express the genes for oxytocin and vasopressin but also how they change with physiological stimulation. With this technique, neurons expressing a gene for luteinizing hormone releasing hormone-like messenger RNA have been discovered in the preoptic area and diagonal bands of the rat forebrain. Seven days of estrogen treatment of ovariectomized female rats increases the LHRH-like messenger RNA in this neuronal system.
原位杂交技术能够检测并测量单个下丘脑神经元中特定的信使核糖核酸，而且在大细胞神经元中已经表明，不仅能确定哪些细胞表达催产素和加压素的基因，还能了解这些基因在生理刺激下如何变化。运用这项技术，在大鼠前脑的视前区和斜角带中发现了表达促黄体生成素释放激素样信使核糖核酸基因的神经元。对切除卵巢的雌性大鼠进行七天的雌激素治疗，可增加该神经元系统中促黄体生成素释放激素样信使核糖核酸的含量。
