Department of Pulmonary Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, People's Republic of China.
Int J Cancer. 2013 Mar 1;132(5):1098-104. doi: 10.1002/ijc.27757. Epub 2012 Aug 24.
Survivin and livin are two members of the inhibitor of apoptosis gene family, which have been found to be expressed in many human cancer tissues. But their expression could not be detected in normal adult tissue. The aim of our study was to evaluate the diagnostic role of survivin and livin mRNA expression in the bronchial aspirates of patients with lung cancer. Seventy lung cancer patients and 26 benign lung disease patients participated in our study. The bronchial aspirates (bronchial wash or bronchoalveolar lavage fluids) obtained during bronchoscopy. Survivin and livin mRNA were determined by reverse transcriptase-polymerase chain reaction. Receiver operating characteristic (ROC) curve was used to analyze diagnostic performance of the two markers. Survivin and livin mRNA levels in patients with lung cancer were significantly higher than in those with benign lung disease (p < 0.001 and p = 0.001, respectively). In lung cancer patients, specimens taken from cancerous bronchi had significantly higher levels of survivin and livin mRNA than specimens from the mirror side bronchi in the same patients (p < 0.001 and p = 0.001, respectively). The best cutoff values of survivin and livin were selected according to ROC curves. The survivin mRNA expression in bronchial aspirates had sensitivity and specificity of 83 and 96% for diagnosis of lung cancer. Livin mRNA detection in bronchial aspirates showed 63% sensitivity and 92% specificity. Our findings suggest that survivin and livin mRNA detection in bronchial aspirates may be valuable diagnostic marker for the early diagnosis of lung cancer.
生存素和 livin 是凋亡抑制基因家族的两个成员，它们已被发现存在于许多人类癌症组织中。但在正常成人组织中无法检测到它们的表达。我们的研究目的是评估 survivin 和 livin mRNA 在肺癌患者支气管抽吸物中的诊断作用。70 例肺癌患者和 26 例良性肺部疾病患者参与了我们的研究。支气管镜检查时获取支气管抽吸物（支气管冲洗液或肺泡灌洗液）。通过逆转录聚合酶链反应测定 survivin 和 livin mRNA。使用接收者操作特性（ROC）曲线分析两个标志物的诊断性能。肺癌患者的 survivin 和 livin mRNA 水平明显高于良性肺部疾病患者（p<0.001 和 p=0.001）。在肺癌患者中，取自癌症支气管的标本中的 survivin 和 livin mRNA 水平明显高于同一患者对侧支气管的标本（p<0.001 和 p=0.001）。根据 ROC 曲线选择 survivin 和 livin 的最佳截断值。支气管抽吸物中 survivin mRNA 的表达对肺癌的诊断具有 83%的敏感性和 96%的特异性。支气管抽吸物中 livin mRNA 的检测显示出 63%的敏感性和 92%的特异性。我们的研究结果表明，支气管抽吸物中 survivin 和 livin mRNA 的检测可能是肺癌早期诊断的有价值的诊断标志物。
