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利用四苯乙烯衍生物的聚集诱导发射特性快速定量奶粉中的酪蛋白。

Rapid casein quantification in milk powder with aggregation induced emission character of tetraphenylethene derivative.

机构信息

CAS Key lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Zhongguancun, Beijing, PR China.

出版信息

Analyst. 2012 Oct 21;137(20):4654-7. doi: 10.1039/c2an35892a.

DOI:10.1039/c2an35892a
PMID:22930455
Abstract

A casein assay has been developed with aggregation induced emission character of BSPOTPE, which is a derivative of tetraphenylethene (TPE). The method exhibits a wide dynamic range of detection for casein from 10 μg ml(-1) to 5000 μg ml(-1), and a good linear range from 20 μg ml(-1) to 1250 μg ml(-1). With sample-pretreatment procedures, we can detect the casein in skimmed and full milk powder, avoiding the interferences from other components and illegal additives in milk.

摘要

已经开发出一种基于 BSPOTPE(四苯乙烯的衍生物)聚集诱导发射特性的酪蛋白检测方法。该方法对酪蛋白的检测范围很宽,从 10μg/ml 到 5000μg/ml,线性范围也很好,从 20μg/ml 到 1250μg/ml。通过样品预处理程序,我们可以检测脱脂奶粉和全脂奶粉中的酪蛋白,避免了牛奶中其他成分和非法添加剂的干扰。

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引用本文的文献

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OFF/ON Red-Emitting Fluorescent Probes for Casein Recognition and Quantification Based on Indolium Derivatives.基于吲哚衍生物的酪蛋白识别和定量的开/关型红色荧光探针。
J Fluoresc. 2017 Jan;27(1):391-398. doi: 10.1007/s10895-016-1967-0. Epub 2016 Nov 17.
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"Off-on" aggregation-based fluorescent sensor for the detection of chloride in water.基于“开-关”聚集的荧光传感器用于检测水中的氯离子。
Org Biomol Chem. 2015 Apr 14;13(14):4266-70. doi: 10.1039/c4ob02409e. Epub 2015 Mar 11.