Chen Kun, Jiang Pu, Deng Shixiong, Wang Nian
Department of Forensic Medicine, Chongqing Medical University, Chongqing, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Aug;32(8):1111-5.
To investigate the expressions of thyroid transcription factor-1 (TTF-1), the type II alveolar epithelial cells (AECII)-specific marker, and vimentin, the fibroblast-specific marker, in the lungs of neonatal mice with bronchopulmonary dysplasia (BPD) and explore the pathogenesis of BPD.
Neonatal mice were exposed to hyperoxia to induce BPD, and pathological changes in the lung tissues were examined. At 3, 7, 14 and 21 days after the exposure, the protein and mRNA expressions of TTF-1 and vimentin were detected by double-labeled immunofluorescence assay and real-time PCR, respectively.
Compared with the neonatal mice exposed in normal air, those with hyperoxic exposure showed developmental disorders and collagen deposition in the lung tissues. The protein expression of TTF-1 decreased while vimentin expression increased in the lung tissues, where their co-expression was observed at 14 and 21 days after the exposure. TTF-1 mRNA expression decreased markedly (P<0.05) and vimentin mRNA increased significantly in the lung tissues at 21 days after hyperoxic exposure of the mice (P<0.05).
Hyperoxia-induced transition of AECII to fibroblasts may play an important role in pulmonary fibrosis in neonatal mice with BPD.
研究支气管肺发育不良（BPD）新生小鼠肺组织中甲状腺转录因子-1（TTF-1，II型肺泡上皮细胞（AECII）特异性标志物）和成纤维细胞特异性标志物波形蛋白的表达情况，探讨BPD的发病机制。
将新生小鼠置于高氧环境中诱导BPD，观察肺组织的病理变化。暴露后3、7、14和21天，分别采用双标免疫荧光法和实时定量PCR检测TTF-1和波形蛋白的蛋白及mRNA表达。
与正常空气环境下的新生小鼠相比，高氧暴露组小鼠肺组织出现发育障碍和胶原沉积。肺组织中TTF-1蛋白表达降低，波形蛋白表达增加，且在暴露后14天和21天观察到二者共表达。小鼠高氧暴露21天后，肺组织中TTF-1 mRNA表达明显降低（P<0.05），波形蛋白mRNA显著增加（P<0.05）。
高氧诱导的AECII向成纤维细胞转变可能在BPD新生小鼠肺纤维化中起重要作用。
