Guo Ying, Liu Yong, Tan Ping-qing, Li Guo, Su Zhong-wu, Tian Yong-quan, Qiu Yuan-zheng
Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Jun;47(6):487-90.
To investigate the mRNA and protein expressions of high mobility group box-1 (HMGB1) in the tumor tissues and sera of patients with laryngeal squamous cell carcinoma (LSCC) and their clinical significance.
Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were used to detected the expressions of HMGB1 mRNA and protein in the tumors and adjacent normal epithelial tissues in 30 patients with LSCC. Serum HMGB1 protein levels in the patients with LSCC and in 10 healthy volunteers were detected with enzyme-linked immunosorbent adsorption experiment (ELISA).
RT-PCR demonstrated that the mean relative mRNA expression levels of HMGB1 (HMGB1/GAPDH) in LSCC tissues and in adjacent normal epithelial tissues were 1.25 ± 0.12 and 0.32 ± 0.04, respectively (t = 40.27, P < 0.05). Western blot revealed that the mean relative protein expression levels of HMGB1 (HMGB1/β-actin) were 1.29 ± 0.10 and 0.34 ± 0.03 (t = 49.84, P < 0.05), respectively. Both mRNA and protein expression levels of HMGB1 were associated with T stage, clinical stage, lymph node metastasis status and smoking (all P < 0.05), but no significant correlation with age, alcohol consumption and primary tumor grade and location (all P > 0.05). Mean serum HMGB1 protein levels in patients with LSCC and healthy volunteers were (24.80 ± 14.08) ng/ml and (23.58 ± 14.69) ng/ml (t = 0.37, P > 0.05).
Both mRNA and protein expressions of HMGB1 were obviously elevated in LSCC, which were associated closely with T stage, clinical stage and lymph node metastasis.
探讨高迁移率族蛋白B1（HMGB1）在喉鳞状细胞癌（LSCC）患者肿瘤组织和血清中的mRNA及蛋白表达情况及其临床意义。
采用半定量逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法检测30例LSCC患者肿瘤组织及癌旁正常上皮组织中HMGB1 mRNA和蛋白的表达。采用酶联免疫吸附试验（ELISA）检测LSCC患者及10名健康志愿者血清中HMGB1蛋白水平。
RT-PCR结果显示，LSCC组织及癌旁正常上皮组织中HMGB1（HMGB1/GAPDH）的平均相对mRNA表达水平分别为1.25±0.12和0.32±0.04（t = 40.27，P < 0.05）。蛋白质印迹法显示，HMGB1（HMGB1/β-肌动蛋白）的平均相对蛋白表达水平分别为1.29±0.10和0.34±（此处原文有误，应为0.03）0.03（t = 49.84，P < 0.05）。HMGB1的mRNA和蛋白表达水平均与T分期、临床分期、淋巴结转移状态及吸烟有关（均P < 0.05），但与年龄、饮酒情况、原发肿瘤分级及部位均无显著相关性（均P > 0.05）。LSCC患者及健康志愿者血清中HMGB1蛋白的平均水平分别为（24.80±14.08）ng/ml和（23.58±14.69）ng/ml（t = 0.37，P > 0.05）。
LSCC中HMGB1的mRNA和蛋白表达均明显升高，且与T分期、临床分期及淋巴结转移密切相关。
