Wan Baoluo, Dong Junhua, Wang Lin, Zhang Sulin, Kong Weijia
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430022, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2011 Jun;25(11):497-500.
To investigate the expression of microtuble-associated protein 1 light chain 3 (LC3) in laryngeal squamous cell carcinoma (LSCC).
The expression of LC3 in 50 cases of LSCC, 45 cases of para-carcinoma, 10 cases of laryngeal papilloma and 16 cases of polyp of vocal cord were detected by immunohistochemistry (MaxVision method). Expression level of LC3 mRNA was assayed by RT-PCR in 41 of LSCC, 41 of para-carcinoma tissue and 11 of polyp of vocal cord.
The positive rates of LC3 protein expression were 60.0%, 93.3%, 90.0%, 93.8% in LSCC tissue, para-carcinoma, laryngeal papilloma and poly of vocal card tissues, respectively. The positive rates of LC3 were significantly lower in LSCC than in para-carcinoma and poly of vocal cord (chi2 = 18.135, P < 0.01). The mRNA levels of LC3 were significantly lower in LSCC than in para-carcinoma and poly of vocal cord (0.57 +/- 0.08 )vs (0.99 +/- 0.11) and (1.07 +/- 0.05) , F = -255.872, P < 0.01. The expression of LC3 were related to tumor location and pathological grade (P < 0.05), but not related to age, T stage, clinical stage and lymphoid metastasis (P > 0.05).
Expression of LC3 are down-regulated in LSCC. The change of autophagic capacity may play an important role in occurrence and development of LSCC.
探讨微管相关蛋白1轻链3（LC3）在喉鳞状细胞癌（LSCC）中的表达情况。
采用免疫组织化学MaxVision法检测50例LSCC、45例癌旁组织、10例喉乳头状瘤及16例声带息肉中LC3的表达。采用RT-PCR法检测41例LSCC组织、41例癌旁组织及11例声带息肉组织中LC3 mRNA的表达水平。
LC3蛋白在LSCC组织、癌旁组织、喉乳头状瘤及声带息肉组织中的阳性表达率分别为60.0%、93.3%、90.0%、93.8%。LSCC中LC3的阳性表达率显著低于癌旁组织及声带息肉（χ2 = 18.135，P < 0.01）。LSCC中LC3的mRNA水平显著低于癌旁组织及声带息肉（0.57±0.08）vs（0.99±0.11）及（1.07±0.05），F = -255.872，P < 0.01。LC3的表达与肿瘤部位及病理分级有关（P < 0.05），但与年龄、T分期、临床分期及淋巴结转移无关（P > 0.05）。
LSCC中LC3表达下调。自噬能力的改变可能在LSCC的发生发展中起重要作用。
